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SetupAttachOpenEndsOperatingManual.SetupAttachOpenEnds HistoryHide minor edits - Show changes to output Added line 5:
'''Note:''' It's best to spray your gloved hands in ethanol before preforming these steps. Changed lines 7-10 from:
[-'''''PERFORM BELLOW STEPS #Take the free #Attach a to:
[-'''''PERFORM BELLOW STEPS USING STERILE TECHNIQUE'''''-] #Take the free female Luer lock connector on the 3" end of the tubing manifold and unwrap the aluminum foil from it #Attach a sterile syringe to the end you just unwrapped Changed line 12 from:
[-'''''PERFORM BELLOW STEPS to:
[-'''''PERFORM BELLOW STEPS USING STERILE TECHNIQUE '''''-] Deleted line 15:
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#If setting up in an incubator make sure to feed the media line through the incubator's port before connecting. Changed line 1 from:
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[[OperatingManual/SetupAssembleClosedSystem | Previous]] Changed lines 17-18 from:
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[[OperatingManual/SetupAssembleClosedSystem | Previous]] [[OperatingManual/SetupAttachMachinery | Next]] Changed line 1 from:
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[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAssembleClosedSystem | Previous]] Added line 1:
[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAssembleClosedSystem | Previous]] [[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAttachMachinery | Next]] Changed line 16 from:
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[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAssembleClosedSystem | Previous]] [[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAttachMachinery | Next]] Changed line 15 from:
[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAttachMachinery | Continue to next section to:
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[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAttachMachinery | Continue to next section: Attach closed system to machinery]] Changed line 15 from:
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[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.SetupAttachMachinery | Continue to next section}} Added lines 1-2:
!!!NEED: Pictures of attaching syringe and media line to octopus tubing Deleted lines 4-5:
[+'''@@PLACING INPUT TUBING INTO PINCH VALVES'''@@+] Changed lines 3-4 from:
#Take free Luer lock connector on the 3" tube #Unwrap a 5 ml?? syringe to:
#Take the free male Luer lock connector on the 3" tube of the octopus tubing system and unwrap the aluminum foil from it #Attach a steril syringe to the unwrapped male Luer lock connector [+'''@@PLACING INPUT TUBING INTO PINCH VALVES'''@@+] Changed line 8 from:
#Take free Luer lock connector on the 4' tube and unwrap the aluminum foil from it to:
#Take free Luer lock connector on the 4' tube (media line) and unwrap the aluminum foil from it Changed line 10 from:
# to:
#Spray ethanol onto both unwrapped connectors Changed lines 1-2 from:
[+'''@@ATTACH STERILE SYRINGE@@'''+] to:
[+'''@@ATTACH STERILE SYRINGE@@'''+]\\ [-'''''PERFORM BELLOW STEPS QUICKLY'''''-] Changed lines 4-6 from:
# [+'''@@ATTACH MEDIA LINE TO MEDIA BOTTLE@@'''+] to:
#Unwrap a 5 ml?? syringe from its packaging and connect it to the unwrapped Luer lock connector [+'''@@ATTACH MEDIA LINE TO MEDIA BOTTLE@@'''+]\\ Changed lines 2-4 from:
#' to:
[-'''''PERFORM BELLOW STEPS QUICKLY''''']- #Take free Luer lock connector on the 3" tube and unwrap the aluminum foil from it #'Unwrap a 5 ml?? syringe from its packaging and connect it to the unwrapped Luer lock connector [+'''@@ATTACH MEDIA LINE TO MEDIA BOTTLE@@'''+] [-'''''PERFORM BELLOW STEPS QUICKLY'''''-] #Take free Luer lock connector on the 4' tube and unwrap the aluminum foil from it #Unrwap the aluminum foil from the output port on the media bottle #Ethanol both unwrapped ends #Connect the Luer lock of the media line to the output port on the media bottle Added lines 1-4:
[+'''@@ATTACH STERILE SYRINGE@@'''+] #Take free Luer lock connector on the 3' tube and unwrap the aluminum foil from it #'''QUICKLY''' unwrap a 5 ml?? syringe from its packaging and connect it to the unwrapped Luer lock connector |