OperatingManual.SetupInnoculate History
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- Repeat for remaining 6 culture vessels
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- Repeat for remaining 6 culture vessels
Previous
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- Prepare a 10 ml?? syringe with a hypodermic needle
- Drip a generous amount of ethanol (2 ml?) on the plug of the culture vessel that will be inoculated
- Uncap liquid cell culture and uptake 5 ml ?? of fluid into the syringe
- Pierce through the plug of culture vessel with the syringe and inject previously uptake fluid into the culture vessel
- Repeat for as many culture vessels as necessary
to:
- Prepare a 10 ml syringe with a hypodermic needle
- Drip a generous amount of ethanol (roughly .5ml) on the plug of the culture vessel that will be inoculated
- Uncap liquid cell culture and uptake 200 uL - 1 ml of fluid into the syringe
- Pierce through the plug of a culture vessel with the syringe and inject half of the fluid into the culture vessel
- Inject the other half of the fluid into another culture vessel
- Repeat for remaining 6 culture vessels
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INNOCULATE
- Prepare a 10 ml?? syringe with a hypodermic needle
- Drip a generous amount of ethanol (2 ml?) on the plug of the culture vessel that will be inoculated
- Uncap liquid cell culture and uptake 5 ml ?? of fluid into the syringe
- Pierce through the plug of culture vessel with the syringe and inject previously uptake fluid into the culture vessel
- Repeat for as many culture vessels as necessary
