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Klavins Lab Turbidostat Wiki | OperatingManual / SetupInnoculate | History
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[+'''@@INNOCULATE@@'''+]
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[+'''@@INOCULATE@@'''+]
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#Repeat for remaining 6 culture vessels
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#Repeat for remaining 6 culture vessels

[[https://students.washington.edu/cnt/pmwiki/pmwiki.php?n=OperatingManual.Software | Previous]]
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#Prepare a 10 ml?? syringe with a hypodermic needle
#Drip a generous amount of ethanol (2 ml?) on the plug of the culture vessel that will be inoculated
#Uncap liquid cell culture and uptake 5 ml ?? of fluid into the syringe
#Pierce through the plug of culture vessel with the syringe and inject previously uptake fluid into the culture vessel
#Repeat for as many culture vessels as necessary
to:
#Prepare a 10 ml syringe with a hypodermic needle
#Drip a generous amount of ethanol (roughly .5ml) on the plug of the culture vessel that will be inoculated
#Uncap liquid cell culture and uptake 200 uL - 1 ml of fluid into the syringe
#Pierce through the plug of a culture vessel with the syringe and inject half of the fluid into the culture vessel
#Inject the other half of the fluid into another culture vessel
#Repeat for remaining 6 culture vessels
Added lines 1-6:
[+'''@@INNOCULATE@@'''+]
#Prepare a 10 ml?? syringe with a hypodermic needle
#Drip a generous amount of ethanol (2 ml?) on the plug of the culture vessel that will be inoculated
#Uncap liquid cell culture and uptake 5 ml ?? of fluid into the syringe
#Pierce through the plug of culture vessel with the syringe and inject previously uptake fluid into the culture vessel
#Repeat for as many culture vessels as necessary
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Page last modified on May 01, 2012, at 07:57 PM