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OperatingManual.SetupPrimePump History
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Changed lines 5-9 from:
- open putty
- select serial under "Connection type"
- select the com port that is attached to the controller (eg "COM7") under "Serial Line"
- Enter 19200 under "Speed"
- click "Open"
to:
- open putty (or the arduino serial monitor)
- select serial under "Connection type" (skip for arduino)
- select the com port that is attached to the controller (eg "COM7") under "Serial Line" (tools->serial port for arduino)
- (select tools->serial monitor in the arduino software)
- Enter 19200 under "Speed" (19200 baud in arduino software)
- click "Open" (skip for arduino)
Changed lines 1-2 from:
to:
Changed lines 31-32 from:
to:
Changed lines 9-10 from:
- you should see a stream of numbmers arrive approximately every 3 seconds, each number should be >100,000 if not something is wrong.
to:
- you should see a stream of numbmers arrive approximately every 3 seconds, each number should be >100,000 if not something is wrong.
Changed lines 5-9 from:
- select serial under "Connection type"
- select the com port that is attached to the controller (eg "COM7") under "Serial Line"
- Enter 19200 under "Speed"
- click "Open"
- you should see a stream of numbmers arrive approximately every 3 seconds, each number should be >100,000 if not something is wrong.
to:
- select serial under "Connection type"
- select the com port that is attached to the controller (eg "COM7") under "Serial Line"
- Enter 19200 under "Speed"
- click "Open"
- you should see a stream of numbmers arrive approximately every 3 seconds, each number should be >100,000 if not something is wrong.
Changed line 9 from:
- you should see a stream of numbmers arrive approximately every 3 seconds
to:
- you should see a stream of numbmers arrive approximately every 3 seconds, each number should be >100,000 if not something is wrong.
Added lines 2-9:
OPEN CONNECTION TO CONTROLLER
- open putty
- select serial under "Connection type"
- select the com port that is attached to the controller (eg "COM7") under "Serial Line"
- Enter 19200 under "Speed"
- click "Open"
- you should see a stream of numbmers arrive approximately every 3 seconds
Added lines 12-19:
turbidostat command list
| Command | Function |
|---|
clo | close all pinch valves |
|---|
sel n | select pinch valve n, where n is in 0-8 (0 is media source) |
|---|
pmv nnnn | move pump to location nnnn where nnnn is in 0-1800 (for most syringes do not exceed 1500) |
|---|
Added lines 1-2:
Changed line 13 from:
to:
Changed lines 9-11 from:
- At this point, check for leaks as your culture vessels are filling. Make sure there aren't any leaks around the inport tubes and tht the number of the valve you opened corresponds to the tube number that you're filling. Also, check to make sure other media inlets aren't leaking (if leaking, pinch valve is broken and will need replacing. Refer to pinch valve replacement)
to:
- At this point, check for leaks as your culture vessels are filling. Make sure there aren't any leaks around the inport tubes and tht the number of the valve you opened corresponds to the tube number that you're filling. Also, check to make sure other media inlets aren't leaking (if leaking, pinch valve is broken and will need replacing. Refer to pinch valve replacement)
Continue to next section
Changed line 9 from:
- At this point, check for leaks as your culture vessels are filling. Make sure there aren't any leaks around the inport tubes and tht the number of the valve you opened corresponds to the tube number that you're filling. Also, check to make sure other media inlets aren't leaking (if leaking, pinch valve is broken and will need replacing. Refer to pinch valve replacement)
to:
- At this point, check for leaks as your culture vessels are filling. Make sure there aren't any leaks around the inport tubes and tht the number of the valve you opened corresponds to the tube number that you're filling. Also, check to make sure other media inlets aren't leaking (if leaking, pinch valve is broken and will need replacing. Refer to pinch valve replacement)
Changed line 9 from:
- At this point, check for leaks as your culture vessels are filling. Make sure there aren't any leaks around the inport tubes and tht the number of the valve you opened corresponds to the tube number that you're filling. Also, check to make sure other media inlets aren't leaking (if leaking, pinch valve is broken and will need replacing. Refer to pinch valve replacement)
to:
- At this point, check for leaks as your culture vessels are filling. Make sure there aren't any leaks around the inport tubes and tht the number of the valve you opened corresponds to the tube number that you're filling. Also, check to make sure other media inlets aren't leaking (if leaking, pinch valve is broken and will need replacing. Refer to pinch valve replacement)
Changed line 9 from:
- At this point, check for leaks as your culture vessels are filling. Make sure there aren't any leaks around the inport tubes and tht the number of the valve you opened corresponds to the tube number that you're filling. Also, check to make sure other media inlets aren't leaking (if leaking, pinch valve is broken and will need replacing. Refer to pinch valve replacement)
to:
- At this point, check for leaks as your culture vessels are filling. Make sure there aren't any leaks around the inport tubes and tht the number of the valve you opened corresponds to the tube number that you're filling. Also, check to make sure other media inlets aren't leaking (if leaking, pinch valve is broken and will need replacing. Refer to pinch valve replacement)
Changed lines 2-5 from:
to:
- Close pinch valves with the controller program
- Unclamp media line originating from media filled carboy
- Remove air from the media line (tapping it works best)
Changed lines 7-9 from:
to:
- One by one, open the valve for each chamber using the controller program
- Use the media solenoid valve manual override to add media to each tube until the level reaches the bottom of the effluent glass capillary
- At this point, check for leaks as your culture vessels are filling. Make sure there aren't any leaks around the inport tubes and tht the number of the valve you opened corresponds to the tube number that you're filling. Also, check to make sure other media inlets aren't leaking (if leaking, pinch valve is broken and will need replacing. Refer to pinch valve replacement)
Changed lines 2-3 from:
to:
- Details coming soon
FILL CHAMBERS WITH MEDIA
- Details coming soon
Added lines 1-3:
PRIME SIPHON
FILL CHAMBERS WITH MEDIA
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