UW Medicine

Invertebrate Longevity and Healthspan

The Invertebrate Longevity and Healthspan Core assists investigators throughout the research community in quantifying a variety of longevity and healthspan measures in two of the major invertebrate model organisms: the budding yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegans. This is accomplished through direct collaboration, training and outreach, and the development and dissemination of new tools and technologies. New technologies we develope and disseminate include microfluidic tools for invertebrate aging studies and the WormBot, a robotic system for high-throughput survival and behavioral phenotyping in C. elegans.  We also work to develop chemostat-based solutions for obtaining age-matched pure populations of yeast for biochemical and cell biological experiments requested by Core users.  The focus on yeast and worms allows us to provide services and technologies to a large number of external investigators and thereby have a correspondingly large impact.  The data generated by this Core can often be correlated with high dimensional computational analyses and with the protein and metabolite phenotypes studied in the other Nathan Shock Center Cores. Invertebrate models are also highly accessible to junior investigators, and our Center pilot projects allow these investigators access to the full range of integrated services provided by the Center Resource Cores.

Services Available:

  • Yeast replicative lifespan analysis
  • Yeast Chronological lifespan analysis
  • Chemostat enrichment of aged yeast populations
  • Yeast growth rate analysis
  • mTOR inhibitor screening
  • Single-cell gene expression and localization during aging
  • Worm lifespan and healthspan analysis and drug screening (manual or WormBot)
  • Transgenic yeast and worm strain construction, including CRISPR
  • Consultation on invertebrate aging study design

Technologies available for dissemination:

  • Fabrication of microfluidic devices for yeast aging studies
  • Fabrication of microfluidic devices for worm imaging
  • The WormBot robotic lifespan and healthspan platform
  • Chemostat devices for enriching aged yeast cells (coming soon)

Core Personnel: The Core is led by Matt Kaeberlein, Maitreya Dunham, and Alex Mendenhall