A Quick Guide to Enzyme Kinetics & Simulations

This is a simple reference guide to enzyme kinetics, for members of the Departments of Medicinal Chemistry and Pharmaceutics of the University of Washington, and for anyone else who finds it useful.

The guide is divided into two parts: the first discusses enzyme kinetics in terms of classical analytical equations - the Michaelis-Menten equation, the Adair-Pauling equation, and so forth; the second part discusses the uses of simulation programs such as Gepasi as applied to enzyme kinetics.

Contents

1. Analytical Equations
1. The Michaelis-Menten and Briggs-Haldane Equations
2. The Quadratic Velocity Equation for Tight-Binding Substrates
3. Multiple Binding: Sequential Binding
4. Multiple Binding: Random-ordered Binding
5. Homotropic Allosterism
6. Inhibition and Activation
7. Translating Between Velocity Equations and Binding Equations
2. Simulations of Enzyme Kinetics
1. Overview of Numerical Simulations and Gepasi
2. Steady-State Simulations
3. Simulating Turnover Kinetics
4. Fitting Simulations to Experimental Data

This guide is a work in progress. Please let me know if you have questions or comments.

- Abhinav Nath (Atkins group), December 2007

This work is licensed under a Creative Commons Attribution 3.0 United States License.