G418 Stock Solution:

80 mg/ml

5 g/ 60ml

2.5 g for 30 ml dH₂O

Filter sterilize and put in fridge.

Nucleotides in Media:

Autoclave:

100x Ade solution = 2.73 g Adenine (Hemisulfate salt)/L dH₂O

100x Ura solution = 2 g Uracil/L dH₂O

Filter Sterilize & Refrigerate:

100x Trp solution = 2 g Tryptophan/ L dH₂O

200x Leu solution = 1.2 g Leucine / 100 ml dH₂O

100x His solution = 0.2 g Histidine / 100 ml dH₂O