Minimal B plates

*Before beginning, see notes below.

1. Yeast nitrogen base w/o aa & AmSO4 2. ammonium sulfate 3. succinic acid 4. NaOH 5. agar 6. Autoclave and cool to 50°C 7. 50% Glucose For B amino acid^+ plates, add after autoclaving 2mg/ml amino acid solution (filter sterilized). For B ad^+ or B ura^+ plates, add after autoclaving 100x adenine or 100x uracil solution. For B tyrp^+ plates, add before autoclaving solid tyrosine.

1 L 1.7 g 5 g 10 g 6 g 20 g 40 ml 5 ml 10 ml 30 mg

1.5 L 2.6 g 7.5 g 15 g 9 g 30 g 60 ml 7.5 ml 15 ml 45 ml