YPD and AT plates

1. Yeast Extract 2. Bactopeptone 3. Agar 4. Autoclave and cool to 50°C. 5. 100x Adenine solution 6. 100x Uracil solution 7. 50% Glucose AT Plates: *Antimycin is toxic, wear gloves* 8. Antimycin dissolved in ethanol, 1 mg/ml.

1 L 10 g 20 g 20 g 10 ml 10 ml 40 ml 1 ml

1.5 L 15 g 30 g 30 g 15 ml 15 ml 60 ml 1.5 ml

2 L 20 g 40 g 40 g 20 ml 20 ml 60 ml 2.0 ml