YPG glycerol plates

1. Yeast Extract 2. Bactopeptone 3. Agar 4. Autoclave and cool to 50°C 5. 100x Adenine solution 6. 100x Uracil solution 7. 50% Glycerol

1 L 10 g 20 g 20 g 10 ml 10 ml 60 ml

1.5 L 15 g 30 g 30 g 15 ml 15 ml 90 ml

2 L 20 g 40 g 40 g 20 ml 20 ml 120 ml

SCG Synthetic Complete Glycerol plates

1. Yeast nitrogen base w/o aa & AmSO4 2. AmSO4 3. Tyrosine 4. NaOH (pellets) 5. Agar 6. Autoclave and cool to 50°C 7. 100x Adenine solution 8. 100x Uracil solution 9. 100x Tryptophan solution 10. 10% CAA 11. 50% glycerol

1 L 1.7 g 5.0 g 30 mg 1 pellet 20 g 10 ml 10 ml 10 ml 40 ml 60 ml

1.5 L 2.6 g 7.5 g 45 mg 2 pellets 30 g 15 ml 15 ml 15 ml 60 ml 90 ml

SCD Synthetic Complete Glucose plates

1. Yeast nitrogen base w/o aa & AmSO4 2. AmSO4 3. Tyrosine 4. NaOH (pellets) 5. Agar 6. Autoclave and cool to 50°C 7. 100x Adenine solution 8. 100x Uracil solution 9. 100x Tryptophan solution 10. 10% CAA 11. 50% Glucose

1 L 1.7 g 5.0 g 30 mg 1 pellet 20 g 10 ml 10 ml 10 ml 40 ml 40 ml

1.5 L 2.6 g 7.5 g 45 mg 2 pellets 30 g 15 ml 15 ml 15 ml 60 ml 60 ml