RECYCLING PLASTIC FROM THE TIP BOXES

 

 

WHAT CAN BE RECYCLED:

 

RED, GREEN AND BLUE INSERTS (flat pieces with 96-holes that come with each stack of tips)

 

ENTIRE EMPTY TIP BOXES AND THEIR LIDS

 

 

WHAT CANNOT BE RECYCLED:

 

CLEAR PLASTIC CONTAINERS THAT THE STACKS COME IN

 

 

WHAT TO DO WITH THE RECYCLABLES:

 

COLLECT IN AN EMPTY BOX (like the ones the stacks of tips arrive in)

 

WHEN BOXES ARE FULL, CONTACT RICHARD ALTOMARE TO ARRANGE A PICKUP

 

richard.altomare@rainin.com

800 828-2788 ext 334

 

 

Daily Checklist

 

1. Glassware

            Take down all dirty lab glassware, pipettes, test tubes, and bottle caps to glass washing in the basement. Pick up clean glassware, etc, and prepare it for autoclaving, or place it in appropriate storage.

 

2. Media Shelf

            We should always have at least the following number of bottles on the shelf:

á      6 of 1L bottles

á      6 of 100ml bottles      

á      2 100ml bottles of drop our solutions (in fridge)

 

3. Plate Media

            Check to see which dry media flasks have been used and what plates are running low. Following guidelines for plates:

á      2 boxes AMP, YPD, URA-, TRP-, URA-TRP-

á      1 box   HIS-, LEU-, ADE-, AT, etc.

 

4. Sterilizing supplies

á      Glassware       30 min.

á      Liquids            30 min.           

á      Used Plates     30 min.

á      Pipettes           20 min.

 

5. Following items will need to be dried in the drying oven after autoclaving

á      Centrifuge bottles and tubes

á      Cortex tubes

á      Sterile eppendorfs in cardboard containers

á      Sterile velvets

á      Pasteur pipettes in glass containers

á      Òexcessively wetÓ glassware

 

6. Autoclaved item distribution- all items should be distributed equally to both labs, except the following:

á      Cortex tubes, drawer under NatalyÕs bench

á      Sterile velvets, drawer over by KenÕs bench

á      Plastic centrifuge tubes, drawer under ValyaÕs bench

 

 

 

 

 

 

 

 

As Needed Checklist

 

1.         Check supplies of stir bars, spatulas, weigh boats. These will usually require washing at least once a week. Scales should also be cleaned weekly.

 

2.         Dirty velvets over by Juan JoseÕs bench. They will need to be washed, dried, sterilized, etc.

 

3.         Used plates in all rooms. These will need to be autoclaved for 30 minutes and put in the dumpster.

 

4.         Check carboys in both labs and also in the cold room. You should have ½ full carboys of 1xTBE in both labs, ¼ full carboy of SDS gel running buffer, 1/8 full carboy of 10xTris/Glycine solution, Western Transfer buffer, and TBS.

 

            Also need to check Ethanol container.

 

5.         Major supplies/media room used: We should always have extra of the following, especially if the Biochemistry Store closes:

á      Plates

á      Paper towels

á      Kim wipes

á      Soap

á      Aluminum foil

á      Yeast extract

á      Tryptone

á      Gloves

á      Eppendorf tubes

á      Weighing paper

á      Filters

á      Yeast nitrogen w/out aa and SO4

á      Bactopeptone

á      Dextrose

á      Saranwrap

á      EDTA

á      Trizma base

á      Boric acid

 

6.         Check the rest of out supplies weekly. We may need to order tubes/flasks, make new plugs, make special orders, etc. ItÕs a good idea to order anything we are fairly low on.

 

 

 

 

 

Supplies Checklist

 

Date:          

      1 box plates

      2 boxes of gloves

      3 boxes of Saranwrap

      6 packages of paper towels

      1 bag of each size Eppendorf tubes

      EDTA

      5 boxes of Kim Wipes

      extra box of weigh paper

      Trizma base

      Soap

      1 full box of each filter size

      Boric Acid

      Aluminum Foil

      Yeast nitrogen w/out aa and SO4

      Yeast extract

      Bactopeptone

      Tryptone

      Dextrose

 

 

 

 

 

 

 

 

 

 

Week:

 

 

Mon

Tues

Wed

Thur

Fri

Take the glassware to the washroom

 

 

 

 

 

Autoclave glassware

 

 

 

 

 

Make liquid media and solutions

 

 

 

 

 

Check carboys in labs and coldroom

 

 

 

 

 

Make plates *

 

 

 

 

 

Weekly**

 

 

 

 

 

 

* There should be two boxes of these plates: AMP, YPD, Ura-, Trp-, and Ura-Trp-

** Check for sterile velvets, sterile toothpicks, and sterile applicators.

 

PREPARATIONS

 

Media

L-Broth

SM

YP

 

 

 

Bacterial

AMP

L-Plates

MMA (aka M9)

 

 

 

Yeast

Ade-

His-

Leu-

Leu-Ura-

Lys-

Trp-

Trp-Leu-

Trp-Ura-

Ura-

 

YPD

YPG

YPD + AT

YPD + 50mM  AA

 

KOAC

Minimal B

 

YT Soft Agar

 

D.O. Solutions

His-

Leu-

Lys-

Trp-Leu-

 

 

 

 

 

 

Tris Buffers

.5M Tris pH 6.8

1M Tris pH 7.5

1M Trus pH 8.0

1M Tris pH 9.0

1.5M Tris pH 8.8

 

Running Buffers

50x TAE

10x TBE

1x TBE

10x Tris/Gly

1x SDS gel

 

Inorganic Solutions

.5M EDTA pH 8.0

3M NaOAc pH 5.2

1M CaCl2

1M MgCl2

3M KCl

M9 Salt Mix

1M LiCl

10% SDS

Sterile H2O

20x SSC

 

Amino Acid Solns.

10% CAA

100x Tryptophan (2g/L)

 

 

 

 

 

 

Pur/Pry Solutions

100x Adenine (2g/L)

100x Uracil (2g/L)

 

 

 

 

 

 

 

 

Carbon Sources

50% Glucose

50% Glycerol

20% Lactate pH 5.5

 

 

 

Western Reagents

1x TBS

Western Transfer Buffer