Sorbitol plates
|
1. sorbitol 2. yeast nitrogen base w/o aa & AmSO4 3. ammonium sulfate 4. tyronsine 5. agar 6. Autoclave and cool to 50¡C 7. 100x Adenine solution 8. 100x Uracil solution 9. 50% glucose |
1 L 218 g 1.7 g 5 g 30 mg 30 g 10 ml 10 ml 40 ml |
1.5 L 327 g 2.6 g 7.5 g 45 mg 45 g 15 ml 15 ml 60 ml |
Sorbitol overlay
|
1. sorbitol 2. yeast nitrogen base w/o aa & AmSO4 3. ammonium sulfate 4. tyronsine 5. agar 6. Autoclave and cool to 50¡C 7. 100x Adenine solution 8. 100x Uracil solution 9. 50% glucose and mix thoroughly 10. aseptically transfer to sterile bottles |
1 L 218 g 1.7 g 5 g 30 mg 30 g 10 ml 10 ml 40 ml |
1.5 L 327 g 2.6 g 7.5 g 45 mg 45 g 15 ml 15 ml 60 ml |