Sporulation plates

1. Potassium Acetate 2. Yeast Extract 3. Glucose 4. Agar 5. Autoclave and cool to 50°C. 6. 100x Amino acids

1 L 10 g 1 g 0.5 g 20 g 10 ml

2 L 20 g 2 g 1 g 40 g 20 ml

100X Amino Acids for sporulation plates

1. Adenine 2. Leucine 3. Lysine 4. Methionine 5. Tryptophan 6. Uracil Filter sterilize and store at 4°C.

for 200 mls 100 mg 150 mg 150 mg 100 mg 100 mg 100 mg