SDS poloyacrylamide gels for Biorad mini-protein
gels
with wide spacers
Separating Gel
|
|
Number of Gels: |
1 |
2 |
3 |
4 |
|
6% gel |
H2O |
4.23 ml |
7.94 ml |
11.64 ml |
15.34 ml |
|
|
30% acrylamide |
1.6 ml |
3.0 ml |
4.4 ml |
5.8 ml |
|
12% gel |
H2O |
2.63 ml |
4.94 ml |
7.24 ml |
9.54 ml |
|
|
30% acrylamide |
3.2 ml |
6.0 ml |
8.8 ml |
11.6 ml |
|
|
1.5 M TRIS pH 8.8 |
2.0 ml |
3.75 ml |
5.5 ml |
7.25 ml |
|
|
10% SDS |
80 ml |
150 ml |
220 ml |
290 ml |
|
|
10% APS |
80 ml |
150 ml |
220 ml |
290 ml |
|
|
TEMED |
8 ml |
15 ml |
22 ml |
29 ml |
Stacking Gel
|
Number of Gels: |
1 |
|
2 |
|
H2O |
2.25 ml |
3.37 ml |
4.50 ml |
|
30% acrylamide |
0.67 ml |
1.0 ml |
1.33 ml |
|
0.5 M TRIS pH6.8 |
1.0 ml |
1.5 ml |
2.0 ml |
|
10% SDS |
40 ml |
60 ml |
80 ml |
|
10% APS |
40 ml |
60 ml |
80 ml |
|
TEMED |
5 ml |
7 ml |
9 ml |