The Bush Lab is a research group in the Department of Chemistry and the Biological Physics, Structure & Design Program at the University of Washington. Our research focuses on the development and application of mass spectrometry and ion mobility spectrometry techniques to elucidate the structures and assembly of protein complexes and subcellular machines.
- Interested in joining the Bush Lab? Click here.
- Our weekly group meetings are held on Tuesdays at 5:10 PM in CHL 068B.
Congratulations to Rae Eaton, who just received a Graduate Fellowship from the American Chemical Society Division of Analytical Chemistry! This award is sponsored by Agilent Technologies.
Structural characterization of small molecular ions by ion mobility mass spectrometry in nitrogen drift gas: improving the accuracy of trajectory method calculations. Jong Wha Lee, Hyun Hee L. Lee, Kimberly L. Davidson, Matthew F. Bush, Hugh I. Kim. Analyst 2018, in press. (Link)
The investigation of ion structures based on a combination of ion mobility mass spectrometry (IM-MS) experiments and theoretical collision cross section (CCS) calculations has become important to many fields of research. However, the accuracy of current CCS calculations for ions in nitrogen drift gas limits the information content of many experiments. In particular, few studies have evaluated and attempted to improve the theoretical tools for CCS calculation in nitrogen drift gas. In this study, Continue reading
Our research on “Fundamental Interactions Between Petroleum Ions and Gases” was featured in the most recent annual report from the American Chemical Society Petroleum Research Fund. Congratulations to Kim Davidson and Anna Bakhtina!
Collision Cross Sections and Ion Structures: Development of a General Calculation Method via High-quality Ion Mobility Measurements and Theoretical Modeling. Jong Wha Lee, Kimberly L. Davidson, Matthew F. Bush, Hugh I. Kim. Analyst 2017, in press. (Link)
Ion mobility mass spectrometry (IM-MS) has become an important tool for the structural investigation of ions in the gas phase. Accurate theoretical evaluation of ion collision cross sections (CCSs) is essential for the effective application of IM-MS in structural studies. However, current theoretical tools have limitations in accurately describing a broad range of ions from small molecules to macromolecules. Continue reading
The Bush Lab welcomes Misa! Click here to learn more about Misa.
Congratulations to Kim Davidson, whose research is featured on the cover of the special issue on “New developments and applications of mass spectrometry methods for studying non-covalent protein interactions”.
Nonspecific Aggregation in Native Electrokinetic Nanoelectrospray Ionization. Kimberly L. Davidson; Derek R. Oberreit; Christopher J. Hogan; Matthew F. Bush. Int. J. Mass Spectrom. 2017, 420, 35–42. (Link | Cover)
Effects of Charge State, Charge Distribution, and Structure on the Ion Mobility of Protein Ions in Helium Gas: Results from Trajectory Method Calculations. Kenneth J. Laszlo, Matthew F. Bush. J. Phys. Chem. A, 2017, in press. (Link)
Collision cross section (Ω) values of gas-phase ions of proteins and protein complexes are used to probe the structures of the corresponding species in solution. Ions of many proteins exhibit increasing Ω-values with increasing charge state but most Ω-values calculated for protein ions have used simple collision models that do not explicitly account for charge. Here we use a combination of ion mobility mass spectrometry experiments with helium gas and trajectory method calculations to characterize the extents to which increases in experimental Ω-values with increasing charge state may be attributed to increased momentum transfer concomitant with enhanced long-range interactions between the protein ion and helium atoms. Continue reading
Matt Bush presented the following talks at the ACS Fall National Meeting in Washington DC, where he also received the Arthur F. Findeis Award for Achievements by a Young Analytical Scientist.
ANYL 269: Interpreting the collision cross sections of proteins: Insights from ion mobility, unfolding, and folding of ions in the gas phase, as a part of the Analytical Division Award Symposium.
PHYS 322: Effects of charge state on the structures of protein ions: Results from cation-to-anion proton-transfer reactions (CAPTR), as a part of the symposium on Gaseous Ion Chemistry & Surface Reactions.
Interpreting the Collision Cross Sections of Native-Like Protein Ions: Insights from Cation-to-Anion Proton-Transfer Reactions. Kenneth J. Laszlo, Matthew F. Bush. Anal. Chem. 2017, DOI: 10.1021/acs.analchem.7b01474. (Link)
The effects of charge state on structures of native-like cations of serum albumin, streptavidin, avidin, and alcohol dehydrogenase were probed using cation-to-anion proton-transfer reactions (CAPTR), ion mobility, mass spectrometry, and complementary energy-dependent experiments. The CAPTR products all have collision cross-section (Ω) values that are within 5.5% of the original precursor cations. The first CAPTR event for each precursor yields products Continue reading