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311T2MLEproj

High resolution imaging of 3 inner hair cells (IHC) innervated by dendrites of the neurons in the spiral ganglion. Calretinin (red) labels the IHC while nerve fibers were labeled for neurofilaments (green). Afferent fibers may be seen extending to the left of the IHC, en route to the outer hair cells and support cells. Nuclei appear blue. Whole mount label embedded in epoxy resin, imaged with widefield microscope, 63x objective (Zeiss) and deconvolved (Huygens software). Glen MacDonald.