DAVID D'ARGENIO
Senior Fellow
University of Washington
HSB, Box K155
Seattle, WA 98195
1-5233
dargenio
Research Interest:
I am characterizing Pseudomonas aeruginosa isolates from chronic lung infections in young children with cystic fibrosis. Traits unique to these isolates are likely to reflect early bacterial adaptations to growth and survival in the lung. Such traits could serve as diagnostic markers to distinguish stages in the progressive lung disease of cystic fibrosis, and may also suggest bacterial vulnerabilities that can be exploited early in the disease.
Publications:
D'Argenio, D. A. and S. I. Miller. 2004. Cyclic di-GMP as a bacterial second messenger. Microbiology 150:2497-2502.
D'Argenio, D. A. 2004. The pathogenic lifestyle of Pseudomonas aeruginosa in model systems of virulence. In J. L. Ramos (ed.), Pseudomonas , vol. I, p.477-503. Kluwer, New York, N.Y.
Ernst, R. K., D. A. D'Argenio, J. K. Ichikawa, M. G. Bangera, S. Selgrade, J. L. Burns, P. Hiatt, K. McCoy, M. Brittnacher, A. Kas, D. H. Spencer, M. V. Olson, B. W. Ramsey, S. Lory, and S. I. Miller. 2003. Genome mosaicism is conserved but not unique in Pseudomonas aeruginosa isolates from the airways of young children with cystic fibrosis. Environ. Microbiol. 5:1341-1349.
Young, D. M., D. A. D'Argenio, M. Jen, D. Parke, and L. N. Ornston. 2003. Gunsalus and Stanier set the stage for selection of cold-sensitive mutants apparently impaired in movement of FAD within 4-hydroxybenzoate hydroxylase. Biochem. Biophys. Res. Commun. 312:153-160.
D'Argenio, D. A., M. W. Calfee, P. B. Rainey, and E. C. Pesci. 2002. Autolysis and autoaggregation in Pseudomonas aeruginosa colony morphology mutants. J. Bacteriol. 184: 6481-6489.
D'Argenio, D. A., L. A. Gallagher, C. A. Berg, and C. Manoil. 2001. Drosophila as a model host for Pseudomonas aeruginosa infection. J. Bacteriol. 183:1466-1471.
Chugani, S. A., M. Whiteley, K. M. Lee, D. D'Argenio, C. Manoil, and E. P. Greenberg. 2001. QscR, a modulator of quorum-sensing signal synthesis and virulence in Pseudomonas aeruginosa . Proc. Natl. Acad. Sci. USA. 98:2752-2757.
D'Argenio, D. A., A. Segura, P. V. Bünz, and L. N. Ornston. 2001. Spontaneous mutations affecting transcriptional regulation by protocatechuate in Acinetobacter . FEMS Microbiol. Lett. 201:15-19.
Young, D. M., D. Parke, D. A. D'Argenio, M. A. Smith, and L. N. Ornston. 2001. Evolution of a catabolic pathway. ASM News 67: 362-369.
Vetting, M. W., D. A. D'Argenio, L. N. Ornston, and D. H. Ohlendorf. 2000. Structure of Acinetobacter strain ADP1 protocatechuate 3,4-dioxygenase at 2.2 Å resolution: implications for the mechanism of an intradiol dioxygenase. Biochemistry. 39:7943-7955.
Parke, D., D. A. D'Argenio, and L. N. Ornston. 2000. Bacteria are not what they eat: that is why they are so diverse. J. Bacteriol. 182:257-263.
D'Argenio, D. A., A. Segura, W. M. Coco, P. V. Bünz, and L. N. Ornston. 1999. The physiological contribution of Acinetobacter PcaK, a transport system that acts upon protocatechuate, can be masked by the overlapping specificity of VanK. J. Bacteriol. 181: 3505-3515.
D'Argenio, D. A., M. W. Vetting, D. H. Ohlendorf, and L. N. Ornston. 1999. Substitution, insertion, deletion, suppression and altered substrate specificity in functional protocatechuate 3,4-dioxygenases. J. Bacteriol. 181:6478-6487.
Segura, A., P. V. Bünz, D. A. D'Argenio, and L. N. Ornston. 1999. Genetic analysis of a chromosomal region containing vanA and -B , genes required for conversion of either ferulate or vanillate to protocatechuate in Acinetobacter . J. Bacteriol. 181:3494-3504.
Kok, R. G., D. A. D'Argenio, and L. N. Ornston. 1998. Mutation analysis of PobR and PcaU, closely related transcriptional activators in Acinetobacter. J. Bacteriol. 180:5058-5069.
Kok, R. G., D. A. D'Argenio, and L. N. Ornston. 1997. Combining localized PCR mutagenesis and natural transformation in direct genetic analysis of a transcriptional regulator gene, pobR . J. Bacteriol. 179:4270-4276.
Gerischer, U., D. A. D'Argenio, and L. N. Ornston. 1996. IS 1236 , a newly discovered member of the IS 3 family, exhibits varied patterns of insertion into the Acinetobacter calcoaceticus chromosome. Microbiology 142:1825-1831.
Creaser, P. C., D. A. D'Argenio, and T. Williams. 1996. Comparative and functional analysis of the AP2 promoter indicates that conserved octamer and initiator elements are critical for activity. Nucleic Acids Res. 24:2597-2605.
