MAIKKE OHLSON
Graduate Student
University of Washington
HSB, Box K116
Seattle, WA 98195
6-5108
mbohlson
Research Interest:
Salmonella enterica serovar Typhimurium infection is initiated by invasion of eukaryotic cells, followed by intracellular replication within a specialized vacuole called the Salmonella-containing vacuole (SCV). The Type III Secretion system (TTSS) encoded on Salmonella-pathogenicity island-2 (SPI-2) translocates effector proteins that promote SCV maturation and intracellular replication. My thesis research focuses on analysis of the SPI-2 TTSS effector SseJ. We hypothesize that SseJ functions to promote the specialized maturation process of the SCV. I am employing a combination of biochemical, genetic, and in vivo mouse model experiments to determine the molecular function of SseJ in order to understand its role during intracellular replication.
Publications:
Ohlson MB , Fluhr K, Birmingham C, Brumell J, Miller SI
SseJ deacylase activity by Salmonella enterica serovar typhimurium promotes virulence in mice. Submitted.
Birmingham CL, Jiang X, Ohlson MB , Miller SI, Brumell JH.
Salmonella-induced filament formation is a dynamic phenotype induced by rapidly replicating Salmonella enterica serovar typhimurium in epithelial cells.
Infect Immun. 2005 Feb;73(2):1204-8.
Voisine C, Cheng YC, Ohlson M , Schilke B, Hoff K, Beinert H,
Marszalek J, Craig EA.
Jac1, a mitochondrial J-type chaperone, is involved in the biogenesis of Fe/S clusters in Saccharomyces cerevisiae.
Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1483-8.
Voisine C, Schilke B, Ohlson M , Beinert H, Marszalek J, Craig EA.
Role of the mitochondrial Hsp70s, Ssc1 and Ssq1, in the maturation of Yfh1.
Mol Cell Biol. 2000 May;20(10):3677-84.
