Biomaterial topography alters healing in vivo and monocyte/macrophage activation in vitro

Citation

Bota, Paige C. S.; Collie, Angela M. B.; Puolakkainen, Pauli; Vernon, Robert B.; Sage, E. Helene; Ratner, Buddy D.; & Stayton, Patrick S. (2010). Biomaterial topography alters healing in vivo and monocyte/macrophage activation in vitro. Journal of biomedical materials research. Part A, 95(2), 649-657.

Abstract

The effect of biomaterial topography on healing in vivo and monocyte/macrophage stimulation in vitro was assessed. A series of expanded polytetrafluoroethylene (ePTFE) materials were characterized by increasing average intranodal distance of 1.2 μm (1.2-ePTFE), 3.0 μm (3.0-ePTFE), and 4.4 μm (4.4-ePTFE), but presented consistent surface chemistry with nonporous PTFE (np-PTFE). Subcutaneous implantation of 4.4-ePTFE into mice resulted in a statistically thinner capsule that appeared less organized and less dense than the np-PTFE response. In vitro, isolated monocytes/macrophages cultured on np-PTFE produced low levels of interleukin 1-beta (IL-1β), 1.2-ePTFE and 3.0-ePTFE stimulated intermediate levels, and 4.4-ePTFE stimulated a 15-fold increase over np-PTFE. Analysis of cDNA microarrays demonstrated that additional proinflammatory cytokines and chemokines, including IL-1β, interleukin 6, tumor necrosis factor alpha, monocyte chemotactic protein 1, and macrophage inflammatory protein 1-beta, were expressed at higher levels by monocytes/macrophages cultured on 4.4-ePTFE at 4 and 24 h, respectively. Expression ratios for several genes were quantified by RT-PCR and were consistent with those from the cDNA array results. These results demonstrate the effect of biomaterial topography on early proinflammatory cytokine production and gene transcription by monocytes/macrophages in vitro and decreased fibrous capsule thickness in vivo.

Keyword(s)

Animals
Biocompatible Materials
Cells, Cultured
Chemokines
Cytokines
Humans
Intercellular Signaling Peptides and Proteins
Macrophage Activation
Macrophages
Male
Materials Testing
Mice
Monocytes
Oligonucleotide Array Sequence Analysis
Polytetrafluoroethylene
Surface Properties

Reference Type

Journal Article

Secondary Title

Journal of biomedical materials research. Part A

Author(s)

Bota, Paige C. S.
Collie, Angela M. B.
Puolakkainen, Pauli
Vernon, Robert B.
Sage, E. Helene
Ratner, Buddy D.
Stayton, Patrick S.

Year Published

2010

Volume Number

95

Issue Number

2

Pages

649-657

ISSN/ISBN

1552-4965

DOI

10.1002/jbm.a.32893