Blood compatibility assessment of polymers used in drug eluting stent coatings

Citation

Szott, L. M.; Irvin, C. A.; Trollsas, M.; Hossainy, S.; & Ratner, B. D. (2016). Blood compatibility assessment of polymers used in drug eluting stent coatings. Biointerphases, 11(2).

Abstract

Differences in thrombosis rates have been observed clinically between different drug eluting stents. Such differences have been attributed to numerous factors, including stent design, injury created by the catheter delivery system, coating application technologies, and the degree of thrombogenicity of the polymer. The relative contributions of these factors are generally unknown. This work focuses on understanding the thrombogenicity of the polymer by examining mechanistic interactions with proteins, human platelets, and human monocytes of a number of polymers used in drug eluting stent coatings, in vitro. The importance for blood interactions of adsorbed albumin and the retention of albumin was suggested by the data. Microscopic imaging and immunostaining enhanced the interpretation of results from the lactate dehydrogenase cell counting assay and provided insight into platelet interactions, total quantification, and morphometry. In particular, highly spread platelets may be surface-passivating, possibly inhibiting ongoing thrombotic events. In many of the assays used here, poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) showed a differentiated protein deposition pattern that may contribute to the explanation of the consistently thromboresistant blood-materials interaction for fluororpolymers cited in literature. These results are supportive of one of several possible factors contributing to the good thromboresistant clinical safety performance of PVDF-HFP coated drug eluting stents. (C) 2016 American Vacuum Society.

Keyword(s)

adsorbed fibrinogen
Adsorption
antibody-binding
carboxy-terminal segment
flow conditions
gamma-chain
glow-discharge
Plasma
platelet-adhesion
von-willebrand-factor

Notes

Dr0ff
Times Cited:4
Cited References Count:58

Reference Type

Journal Article

Secondary Title

Biointerphases

Author(s)

Szott, L. M.
Irvin, C. A.
Trollsas, M.
Hossainy, S.
Ratner, B. D.

Year Published

2016

Date Published

1464739200

Volume Number

Issue Number

ISSN/ISBN

1934-8630

DOI

Artn 029806
10.1116/1.4944586