Engineered polymer-media interfaces for the long-term self-renewal of human embryonic stem cells

Citation

Irwin, Elizabeth F.; Gupta, Rohini; Dashti, Derek C.; & Healy, Kevin E. (2011). Engineered polymer-media interfaces for the long-term self-renewal of human embryonic stem cells. Biomaterials, 32(29), 6912-6919.

Abstract

We have developed a synthetic polymer interface for the long-term self-renewal of human embryonic stem cells (hESCs) in defined media. We successfully cultured hESCs on hydrogel interfaces of aminopropylmethacrylamide (APMAAm) for over 20 passages in chemically-defined mTeSR™1 media and demonstrated pluripotency of multiple hESC lines with immunostaining and quantitative RT-PCR studies. Results for hESC proliferation and pluripotency markers were both qualitatively and quantitatively similar to cells cultured on Matrigel™-coated substrates. Mechanistically, it was resolved that bovine serum albumin (BSA) in the mTeSR™1 media was critical for cell adhesion on APMAAm hydrogel interfaces. This study uniquely identified a robust long-term culture surface for the self-renewal of hESCs without the use of biologic coatings (e.g., peptides, proteins, or Matrigel™) in completely chemically-defined media that employed practical culturing techniques amenable to clinical-scale cell expansion.

Keyword(s)

Acrylamides
Adsorption
Animals
Biocompatible Materials
Cattle
Cell Culture Techniques
Cell Differentiation
Cell Proliferation
Cells, Cultured
Culture Media
Embryonic Stem Cells
Humans
Hydrogels
Materials Testing
Polymers
Serum Albumin, Bovine
Surface Properties

Reference Type

Journal Article

Secondary Title

Biomaterials

Author(s)

Irwin, Elizabeth F.
Gupta, Rohini
Dashti, Derek C.
Healy, Kevin E.

Year Published

2011

Volume Number

32

Issue Number

29

Pages

6912-6919

ISSN/ISBN

1878-5905

DOI

10.1016/j.biomaterials.2011.05.058