Citation
Samuel, N. T.; Lee, C. Y.; Gamble, L. J.; Fischer, D. A.; & Castner, D. G. (2006).
NEXAFS characterization of DNA components and molecular-orientation of surface-bound DNA oligomers.
Journal of Electron Spectroscopy and Related Phenomena, 152(3), 134-142.
Abstract
Single stranded DNA oligomers (ssDNA) immobilized onto solid surfaces forms the basis for several biotechnological applications such as DNA microarrays, affinity separations, and biosensors. The surface structure of the surface-bound oligomers is expected to significantly influence their biological activity and interactions with the environment. In this study near-edge X-ray absorption fine structure spectroscopy (NEXAFS) is used to characterize the components of DNA (nucleobases, nucleotides and nucleosides) and the orientation information of surface-bound ssDNA. The K-edges of carbon, nitrogen and oxygen have spectra with features that are characteristic of the different chemical species present in the nucleobases of DNA. The effect of addition of the DNA sugar and phosphate components on the NEXAFS K-edge spectra was also investigated. The polarization-dependent nitrogen K-edge NEXAFS data show significant changes for different orientations of surface bound ssDNA. These results establish NEXAFS as a powerful technique for chemical and structural characterization of surface-bound DNA oligomers. (c) 2006 Elsevier B.V. All rights reserved.
Keyword(s)
absorption fine-structureamino-acidsDNAgene-expression patternsgold surfacesimmobilizationk-edgemonolayersnexafssingle-stranded-DNAspectraspectroscopyx-rayxps
Reference Type
Journal Article
Secondary Title
Journal of Electron Spectroscopy and Related Phenomena
Author(s)
Samuel, N. T.Lee, C. Y.Gamble, L. J.Fischer, D. A.Castner, D. G.
Year Published
2006
Date Published
1151712000
Volume Number
152
Issue Number
3
Pages
134-142
DOI
DOI 10.1016/j.elspec.2006.04.004
