Citation
Grunkemeier, J. M.; Tsai, W. B.; McFarland, C. D.; & Horbett, T. A. (2000).
The effect of adsorbed fibrinogen, fibronectin, von Willebrand factor and vitronectin on the procoagulant state of adherent platelets.
Biomaterials, 21(22), 2243-2252.
Abstract
Procoagulant (activated) platelets provide a site for assembly of the prothrombinase complex which can rapidly convert prothrombin into thrombin (a potent inducer of clot formation). Previously, we reported that adhesion of platelets to surfaces preadsorbed with blood plasma caused them to become procoagulant. In the present study we investigated the effect of adsorbed adhesion proteins (fibrinogen (Fg), fibronectin (Fn), von Willebrand factor (vWF) and vitronectin (Vn)) on the procoagulant activity of adherent platelets. Adsorbed Fn, vWF and Fg promoted platelet adhesion in the following order: Fn < vWF = Fg. However, these proteins promoted platelet activation (thrombin generation per adherent platelet) in the following order: Fg < Fn < vWF. Adsorption with a series of dilutions of normal plasma, serum, and plasmas deficient in or depleted of von Willebrand factor (de-vWF), fibronectin (de-Fn), vitronectin (de-Vn), or both vitronectin and fibronectin (de-VnFn) resulted in varied platelet adhesion, but little difference in platelet activation. However, preadsorption with dilute de-vWF plasma induced lower procoagulant activity than normal plasma. Preadsorption with normal plasma resulted in higher levels of platelet activation than preadsorption with Fg, suggesting that adsorption of plasma proteins other than Fg caused the high levels of activation observed for plasma preadsorbed surfaces.
Keyword(s)
AdsorptionBlood PlateletsFibrinogenFibronectinsHumansplatelet activationPlatelet AdhesivenessThrombinVitronectinvon Willebrand Factor
Reference Type
Journal Article
Secondary Title
Biomaterials
Author(s)
Grunkemeier, J. M.Tsai, W. B.McFarland, C. D.Horbett, T. A.
Year Published
2000
Volume Number
21
Issue Number
22
Pages
2243-2252
