Three-dimensional localization of polymer nanoparticles in cells using ToF-SIMS

Citation

Graham, D. J.; Wilson, J. T.; Lai, J. J.; Stayton, P. S.; & Castner, D. G. (2016). Three-dimensional localization of polymer nanoparticles in cells using ToF-SIMS. Biointerphases, 11(2).

Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) three-dimensional (3D) depth profiling and a novel background subtraction method were used to localize polymeric nanoparticles within cells. Results showed that ToF-SIMS 3D depth profiling is capable of localizing polymer nanoparticles within HeLa cells. ToF-SIMS results compared well with optical images of cells incubated with fluorescently labeled polymer nanoparticles, with both imaging techniques demonstrating clustering of nanoparticles in punctate regions consistent with endosomal localization as anticipated based on the nanoparticle design. (C) 2015 American Vacuum Society.

Keyword(s)

copolymer
design
intracellular delivery
micelle carriers
sirna delivery
therapeutics
trafficking

Notes

Dr0ff
Times Cited:5
Cited References Count:24

Reference Type

Journal Article

Secondary Title

Biointerphases

Author(s)

Graham, D. J.
Wilson, J. T.
Lai, J. J.
Stayton, P. S.
Castner, D. G.

Year Published

2016

Date Published

1464739200

Volume Number

11

Issue Number

2

ISSN/ISBN

1934-8630

DOI

Artn 02a304
10.1116/1.4934795