Citation
Reed, Jamie A.; Lucero, Adrianne E.; Hu, Steve; Ista, Linnea K.; Bore, Mangesh T.; López, Gabriel P.; & Canavan, Heather E. (2010).
A Low-Cost, Rapid Deposition Method for “Smart” Films: Applications in Mammalian Cell Release.
ACS Applied Materials & Interfaces, 2(4), 1048-1051.
Abstract
The ?smart? polymer poly (N-isopropyl acrylamide), or pNIPAM, has been studied for bioengineering applications. The polymer?s abrupt change in hydrophobicity near physiologic temperatures makes it ideal for use as a substrate in many applications, including protein separation and prevention of biofouling. To tether pNIPAM, many techniques such as plasma deposition, have been utilized, but most are expensive and require long equipment calibration or fabrication periods. Recently, a novel method for codepositing this smart polymer with a sol?gel, tetraethyl orthosilicate (TEOS), was developed. In this work, we adapt this technique for applications in mammalian cell attachment/detachment. In addition, we compare the effects of the pNIPAM/TEOS ratio to functionality using surface analysis techniques (XPS and contact angles). We found the optimal ratio to be 0.35 wt % pNIPAM/TEOS. Cell detachment from these substrates indicate that they would be ideal for applications that do not require intact cell sheets, such as biofouling prevention and protein separation, as this technique is a simple and affordable technique for pNIPAM deposition.
Reference Type
Journal Article
Secondary Title
ACS Applied Materials & Interfaces
Author(s)
Reed, Jamie A.Lucero, Adrianne E.Hu, SteveIsta, Linnea K.Bore, Mangesh T.López, Gabriel P.Canavan, Heather E.
Year Published
2010
Date Published
1272412800
Volume Number
2
Issue Number
4
Pages
1048-1051
ISSN/ISBN
1944-8244
DOI
A Low-Cost, Rapid Deposition Method for “Smart” Films10.1021/am900821t
