Citation
Zorn, G.; Castner, D. G.; Tyagi, A.; Wang, X.; Wang, H.; & Yan, M. (2015). Analysis of the surface density and reactivity of perfluorophenylazide and the impact on ligand immobilization. J Vac Sci Technol A, 33(2), 021407. PMCID: 4327916Abstract
Perfluorophenylazide (PFPA) chemistry is a novel method for tailoring the surface properties of solid surfaces and nanoparticles. It is general and versatile, and has proven to be an efficient way to immobilize graphene, proteins, carbohydrates, and synthetic polymers. The main thrust of this work is to provide a detailed investigation on the chemical composition and surface density of the PFPA tailored surface. Specifically, gold surfaces were treated with PFPA-derivatized (11-mercaptoundecyl)tetra(ethylene glycol) (PFPA-MUTEG) mixed with 2-[2-(2-mercaptoethoxy)ethoxy]ethanol (MDEG) at varying solution mole ratios. Complementary analytical techniques were employed to characterize the resulting films including Fourier transform infrared spectroscopy to detect fingerprints of the PFPA group, x-ray photoelectron spectroscopy and ellipsometry to study the homogeneity and uniformity of the films, and near edge x-ray absorption fine structures to study the electronic and chemical structure of the PFPA groups. Results from these studies show that the films prepared from 90:10 and 80:20 PFPA-MUTEG/MDEG mixed solutions exhibited the highest surface density of PFPA and the most homogeneous coverage on the surface. A functional assay using surface plasmon resonance with carbohydrates covalently immobilized onto the PFPA-modified surfaces showed the highest binding affinity for lectin on the PFPA-MUTEG/MDEG film prepared from a 90:10 solution.Notes
Zorn, GiladCastner, David G
Tyagi, Anuradha
Wang, Xin
Wang, Hui
Yan, Mingdi
P41 EB002027/EB/NIBIB NIH HHS/
R01 GM080295/GM/NIGMS NIH HHS/
R15 GM066279/GM/NIGMS NIH HHS/
J Vac Sci Technol A. 2015 Mar;33(2):021407. Epub 2015 Feb 12.