Covalent Coupling and Characterization of Supported Lipid Layers

Citation

McArthur, Sally L.; Halter, Michael W.; Vogel, Viola; & Castner, David G. (2003). Covalent Coupling and Characterization of Supported Lipid Layers. Langmuir, 19(20), 8316-8324.

Abstract

To investigate protein and cell surface interactions, robust and increasingly complex model surfaces need to be developed to mimic specific aspects of the cell membrane structure. Polymer-supported lipid monolayers and bilayers present an opportunity to develop rugged cell mimetic surfaces that may be stable in a wide range of in vivo and in vitro applications and characterization techniques. We have investigated the stability of dimyristoylphosphatidylethanolamine (DMPE) monolayers grafted to poly(hydroxyethyl methacrylate) (pHEMA) via 1,1?-carbonyldiimidazole (CDI) chemistry using X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectrometry (ToF-SIMS), and fluorescence microscopy. The results illustrate that it is possible to covalently couple the amine-containing lipid headgroup to the carboxyl and hydroxyl groups of the pHEMA and retain a proportion of the lipids at the surface after the samples are sonicated in ethanol. Most importantly, the cross-linking efficiency and retention of the resulting lipid layer is higher if the lipid is transferred to CDI-activated HEMA by Langmuir?Blodgett deposition rather than by adsorption of the lipid directly from solution. Both factors are critical if these monolayers are to form a stable and reproducible model system.

Reference Type

Journal Article

Secondary Title

Langmuir

Author(s)

McArthur, Sally L.
Halter, Michael W.
Vogel, Viola
Castner, David G.

Year Published

2003

Date Published

1062374400

Volume Number

Issue Number

Pages

8316-8324

DOI

10.1021/la026928h