Effect of polymer deposition method on thermoresponsive polymer films and resulting cellular behavior

Citation

Reed, J. A.; Love, S. A.; Lucero, A. E.; Haynes, C. L.; & Canavan, H. E. (2012). Effect of polymer deposition method on thermoresponsive polymer films and resulting cellular behavior. Langmuir: the ACS journal of surfaces and colloids, 28(4), 2281-2287.

Abstract

Poly(N-isopropyl acrylamide) or pNIPAM is a thermoresponsive polymer that is widely studied for use in bioengineering applications. The interest in this polymer lies in the polymer's unique capability to undergo a sharp property change near physiological temperature, which aids in the spontaneous release of biological cells from substrates. Currently, there are many methods for depositing pNIPAM onto substrates, including atom-transfer radical polymerization (ATRP) and electron beam ionization. Each method yields pNIPAM-coated substrates with different surface characteristics that can influence cell behavior. In this work, we compare two methods of pNIPAM deposition: plasma deposition and codeposition with a sol-gel. The resulting pNIPAM films were analyzed for use as substrates for mammalian cell culture based on surface characterization (XPS, ToF-SIMS, AFM, contact angles), cell attachment/detachment studies, and an analysis of exocytosis function using carbon-fiber microelectrode amperometry (CFMA). We find that although both methods are useful for the deposition of functional pNIPAM films, plasma deposition is much preferred for cell-sheet engineering applications because of the films' thermoresponse, minimal change in cell density, and maintenance of supported cell exocytosis function.

Keyword(s)

Acrylic Resins
Animals
Cattle
Cell Survival
Chromaffin Cells
Endothelial Cells
Exocytosis
Mice
Microelectrodes
Plasma Gases
Polymerization
Surface Properties
Temperature

Reference Type

Journal Article

Secondary Title

Langmuir: the ACS journal of surfaces and colloids

Author(s)

Reed, J. A.
Love, S. A.
Lucero, A. E.
Haynes, C. L.
Canavan, H. E.

Year Published

2012

Date Published

1327968000

Volume Number

Issue Number

Pages

2281-2287

ISSN/ISBN

1520-5827

DOI

10.1021/la102606k