Enhanced in-vitro blood compatibility of 316L stainless steel surfaces by reactive landing of hyaluronan ions

Citation

Volný, Michael; Elam, W. Timothy; Ratner, Buddy D.; & Turecek, Frantisek (2007). Enhanced in-vitro blood compatibility of 316L stainless steel surfaces by reactive landing of hyaluronan ions. Journal of biomedical materials research. Part B, Applied biomaterials, 80(2), 505-510.

Abstract

A novel dry process for immobilization of hyaluronan on stainless steel surfaces is presented. This process that we call reactive landing is based on an interaction of hyperthermal gas-phase hyaluronan ions with plasma-cleaned and activated stainless steel surfaces. Reactive landing is performed on a unique instrument that combines an in-situ plasma reactor with an electrospray ion source and ion transfer optics. Gas-phase hyaluronan anions are obtained by electrospray ionization of sodium hyaluronan solutions and immobilized by reactive landing on large-area stainless steel surfaces. The immobilized hyaluronan withstands extensive washing with polar solvents and solutions, and the washed surfaces maintain the protective properties against blood platelet activation. The mechanism of hyaluronan discharge and immobilization is discussed.

Keyword(s)

Biocompatible Materials
Coated Materials, Biocompatible
Humans
Hyaluronic Acid
Ions
Materials Testing
Microscopy, Electron, Scanning
platelet activation
Platelet Adhesiveness
Spectrum analysis
Stainless Steel
Surface Properties
X-Rays

Reference Type

Journal Article

Secondary Title

Journal of biomedical materials research. Part B, Applied biomaterials

Author(s)

Volný, Michael
Elam, W. Timothy
Ratner, Buddy D.
Turecek, Frantisek

Year Published

2007

Volume Number

80

Issue Number

2

Pages

505-510

ISSN/ISBN

1552-4973

DOI

10.1002/jbm.b.30624