Citation
Kirk, James T.; Brault, Norman D.; Baehr-Jones, Tom; Hochberg, Michael; Jiang, Shaoyi; & Ratner, Daniel M. (2013).
Zwitterionic polymer-modified silicon microring resonators for label-free biosensing in undiluted human plasma.
Biosens Bioelectron, 42, 100-105.
Abstract
A widely acknowledged goal in personalized medicine is to radically reduce the costs of highly parallelized, small fluid volume, point-of-care and home-based diagnostics. Recently, there has been a surge of interest in using complementary metal-oxide-semiconductor (CMOS)-compatible silicon photonic circuits for biosensing, with the promise of producing chip-scale integrated devices containing thousands of orthogonal sensors, at minimal cost on a per-chip basis. A central challenge in biosensor translation is to engineer devices that are both sensitive and specific to a target analyte within unprocessed biological fluids. Despite advances in the sensitivity of silicon photonic biosensors, poor biological specificity at the sensor surface remains a significant factor limiting assay performance in complex media (i.e. whole blood, plasma, serum) due to the non-specific adsorption of proteins and other biomolecules. Here, we chemically modify the surface of silicon microring resonator biosensors for the label-free detection of an analyte in undiluted human plasma. This work highlights the first application of a non-fouling zwitterionic surface coating to enable silicon photonic-based label-free detection of a protein analyte at clinically relevant sensitivities in undiluted human plasma.
Keyword(s)
Amino Acid Transport Systems, NeutralBiosensing TechniquesHumansPolymersSemiconductorsSerumsilicon
Reference Type
Journal Article
Secondary Title
Biosens Bioelectron
Author(s)
Kirk, James T.Brault, Norman D.Baehr-Jones, TomHochberg, MichaelJiang, ShaoyiRatner, Daniel M.
Year Published
2013
Date Published
1365984000
Volume Number
42
Pages
100-105
ISSN/ISBN
1873-4235
DOI
10.1016/j.bios.2012.10.079
