The UW NAPE Center will host a free, week-long imaging workshop focusing on deep-brain single- and multi-photon functional imaging this fall (Sept. 15-19, 2025).
Registration is due July 28, 2025.
This workshop will instruct attendees on the entire pipeline for deep-brain calcium imaging experimentation focusing on the following steps:
Overall calcium imaging study design (Mickey Ruiz)
Behavioral setup and integration with imaging (Drs. Adam Gordon-Fennell, Raaj Gowrishankar)
Introduction to viral and genetic tools (Dr. Selena Schattauer; NAPE Molecular Genetics Resource Core)
Virus injection, GRIN lens, and headcap implantation surgery (Dr. Sean Piantadosi, Abi Elerding)
Photometry (Amanda Pasqualini, Cat Zamarano)
Imaging acquisition using 1-photon Inscopix mini-microscope (Dr. Peter Schuette, Bruker; Dr. Sekun Park)
Imaging acquisition using 2-photon microscopy (Drs. Charles Zhou, Raaj Gowrishankar, and Madison Martin)
Biosensors (Dr. Carlie Neiswanger and Dr. Justin Lee)
Data preprocessing and analysis (Drs. Abbie Schindler, Charles Zhou, Weston Fleming)
Data post-processing and analysis (Lucy Tian)
Generalized linear models for time-series and behavior (Ethan Ancell)
Discussion and brainstorming of personalized attendee study design
When: September 15-19, 2025, 9am to 2 or 3pm each day with lunch break
Where: University of Washington Magnuson Health Science Building J-wing and K-wing lab and meeting space. We will host a limited number of attendees for the hands-on surgery component. Others are invited to join the didactic portions in-person.
Who: We are prioritizing Graduate Students and Post Docs who have minimal to no experience in 1-photon or 2-photon calcium imaging.
Contact: Feel free to reach out to Dr. Charles Zhou (NAPE Imaging Core Director; zhouzc@uw.edu), Dr. Lydia Gordon-Fennell (NAPE Program Manager; lgordonf@uw.edu), or Dr. Lusine Eyde (NAPE Program Manager; Lusineg@uw.edu) with any questions.