Imaging & Genetics Workshop

The UW NAPE Center will host a week-long imaging workshop focusing on deep-brain single- and multi-photon functional imaging this fall (Oct. 21-25, 2024).

Registration: TBA

This workshop will instruct attendees on the entire pipeline for deep-brain calcium imaging experimentation focusing on the following steps:

• Overall calcium imaging study design (Cat Zamorano)

• Behavioral setup and integration with imaging (Drs. Adam Gordon-Fennell, Raaj Gowrishankar)

• Introduction to viral and genetic tools (Dr. Selena Schattauer; NAPE Molecular Genetics Resource Core)

Virus injection, GRIN lens, and headcap implantation surgery (Dr. Sean Piantadosi)

Photometry (Dr. Adam Gordon-Fennell and Carrie Stine)

Imaging acquisition using 1-photon Inscopix mini-microscope (Dr. Peter Schuette, Bruker)

Imaging acquisition using 2-photon microscopy (Drs. Charles Zhou, Raaj Gowrishankar, and Madison Martin)

Biosensors (Dr. Carlie Neiswanger and Justin Lee)

Data preprocessing and analysis (Drs. Abbie Schindler, Charles Zhou, Weston Fleming)

Data post-processing and analysis (Drs. Charles Zhou and Ethan Ancell)

Discussion and brainstorming of personalized attendee study design

When: October 21-25, 2024, 9am to 2 or 3pm each day with lunch break

Where: University of Washington Magnuson Health Science Building J-wing and K-wing lab and meeting space. We will host a limited number of attendees for the hands-on surgery component. Others are invited to join the didactic portions virtually or in-person.

Who: We are prioritizing Graduate Students and Post Docs who have minimal to no experience in 1-photon or 2-photon calcium imaging.

Contact: Feel free to reach out to Dr. Charles Zhou (NAPE Imaging Core Director; zhouzc@uw.edu) or Dr. Lusine Eyde (NAPE Program Manager; Lusineg@uw.edu) with any questions.