Training Requirements:

Listed below are the training requirements personnel need to complete in order to work with animals at the University of Washington. For a full listing of the Training Program's course offerings, please visit the Online Courses and In Person Courses pages. To view your training record, please visit My Research Training Transcript

All individuals working with animals must complete the following:

  • Online Laws and Regulations training. This training must be updated every 5 years.
  • Animal Use Medical Screening (AUMS). Screening needs to be updated as per the OHN's recommendations. Please reference the instructions listed on the EH&S website for further information.
  • Submit a New Personnel form to be added to your PI's protocol. Please reference the instructions listed on the form for further information.

Individuals who will be handling or performing procedures on awake rodents, ferrets, rabbits, pigs, or dogs must complete the appropriate training class(es) before beginning work with each species. Such personnel must have completed or be registered for the appropriate species-specific training class before being added to a protocol. Specifically:

  • Individuals working with mice must complete the Mouse Hands-on Laboratory. Prerequisite: Completion of the online “Working with Mice at the UW” course.
  • Individuals working with rats must complete the Rat Hands-on Laboratory. Prerequisite: Completion of the online “Working with Rats at the UW” course.
  • Individuals working with gerbils must complete the Gerbil Hands-on Laboratory. No prerequisites.
  • Individuals working with hamsters must complete the Hamster Hands-on Laboratory. No prerequisites.
  • Individuals working with guinea pigs must complete the Guinea Pig Hands-on Laboratory. No prerequisites.
  • Individuals working with ferrets must complete the Ferret Hands-on Laboratory. No prerequisites.
  • Individuals working with rabbits must complete the Rabbit Hands-on Laboratory. No prerequisites.
  • Individuals working with pigs must complete the Pig Hands-on Laboratory. No prerequisites.
  • Individuals working with dogs must complete the Dog Hands-on Laboratory. No prerequisites.

    Personnel who are working with, or who have worked with, rabbits or pigs at the UW prior to the time of adoption of this policy (May 17, 2012) are exempt from the requirement to complete the rabbit or pig training class, respectively. Personnel who are working with, or who have worked with, dogs at the UW prior to October 6, 2014 are exempt from the requirement to complete the dog training class.

For individuals working within centralized Department of Comparative Medicine vivaria:

  • If you are a rodent user, you will be required to complete the online Specific Pathogen Free (SPF) training on an annual basis.
  • Individuals (both rodent and non-rodent users) who need to enter centralized vivaria must complete the Facility Orientation for the appropriate facility prior to initial entry approval. Prerequisites: Must be listed on a UW protocol. Rodent Users must complete the SPF training.
  • Individuals who will work in Animal Bio-Safety Level 2 or 3 rooms within centralized vivaria must complete the Animal Bio-Safety Level 2 or 3 Room Orientation . Prerequisite: Completion of the facility orientation.
  • Individuals who will work in a Helicobacter free room must complete the Helicobacter Free Room Training. Prerequisite: Completion of the facility orientation.
  • Individuals who will work in the Gnotobiotic Animal Core (GNAC) must complete the Gnotobiotic Animal Core (GNAC) Orientation. Prerequisite: Completion of the facility orientation.

For individuals working within decentralized laboratory-managed vivaria (i.e., not Comparative Medicine run):

Aquatics:

Rodents:

  • Individuals who provide husbandry and care for their own rodents must complete the Decentralized Animal Care and Records class and the Sick Rodent Recognition class (Note: these classes are offered back to back). The PI or a professional staff lab manager is also required to complete both classes.

Species other than aquatics and rodents:

  • Individuals who provide husbandry and care for their own animals (non-aquatic and non-rodent) must complete the Decentralized Animal Care and Records class. The PI or a professional staff lab manager is also required to complete this class.

For individuals performing aseptic animal surgery for the first time at the UW, as well as UW personnel performing aseptic surgery on a new category of animal (rodents; USDA-covered non-rodents; other animals):

Rodent users (e.g., mice, rats, gerbils, hamsters, guinea pigs, etc.):

  • All personnel must complete the online Introduction to Rodent Surgery course.
  • All personnel* must complete the in-person courses Surgery Laboratory Part IA and Surgery Laboratory Part II. Prerequisite: Completion of the online “Introduction to Rodent Surgery” course.
  • All personnel must complete Supervised Surgery Experience within their lab which is supervised by trained IACUC protocol staff in protocol-approved surgery location(s) until the individual is ready for certification. Prerequisites: Completion of the “Surgery Laboratory Part II” course.
  • All personnel performing aseptic surgery independently must complete Aseptic Surgery Certification which involves on-site observation by the Attending Veterinarian or a designee. Based on the on-site observation, personnel are either certified to perform aseptic surgery independently, or required to have additional training in specific aspects of surgery before certification is granted. Prerequisite: Completion of Supervised Surgery Experience.

USDA-covered non-rodent users (e.g., bats, rabbits, cats, ruminants, dogs, pigs, ferrets, non-human primates, etc.):

  • All personnel* must complete the online Introduction to Surgery (Non-Rodent, USDA-Covered Animals) course.
  • All personnel* must complete the in-person courses Surgery Laboratory Part IB and Surgery Laboratory Part II. Prerequisite: Completion of the online “Introduction to Surgery (Non-Rodent, USDA-Covered Animals) ” course.
  • All personnel must complete Supervised Surgery Experience within their lab which is supervised by trained IACUC protocol staff in protocol-approved surgery location(s) until the individual is ready for certification. Prerequisites: Completion of the “Surgery Laboratory Part II” course.
  • All personnel performing aseptic surgery independently must complete Aseptic Surgery Certification which involves on-site observation by the Attending Veterinarian or a designee. Based on the on-site observation, personnel are either certified to perform aseptic surgery independently, or required to have additional training in specific aspects of surgery before certification is granted. Prerequisite: Completion of Supervised Surgery Experience.

Other animals (e.g., fish, frogs, birds, etc.):

  • Course requirements are determined on a case-by-case basis.
  • All personnel must complete Supervised Surgery Experience within their lab which is supervised by trained IACUC protocol staff in protocol-approved surgery location(s) until the individual is ready for certification.
  • All personnel performing aseptic surgery independently must complete Aseptic Surgery Certification which involves on-site observation by the Attending Veterinarian or a designee. Based on the on-site observation, personnel are either certified to perform aseptic surgery independently, or required to have additional training in specific aspects of surgery before certification is granted. Prerequisite: Completion of Supervised Surgery Experience.

*Exceptions:

  • If you are a surgeon (MD, DVM) with extensive surgery experience and would like to petition out of the laboratory classes, please email auts@uw.edu.
  • USDA-covered non-rodent users:Personnel assisting with surgery in an OR in Comparative Medicine with a veterinarian/vet tech looking over their shoulder may be exempted from the online and laboratory classes. If this exception applies to you, please email auts@uw.edu.
  • Bat, rabbit, cat, ruminant, dog, pig, ferret users: Medical students who are receiving aseptic surgery instruction as part of their schooling may be exempted from the online and laboratory classes. If this exemption applies to you, please email auts@uw.edu.

Certification is required for individuals performing the following procedures:

Mice:

  • Cervical dislocation (anesthetized or unanesthetized)
  • Retro-orbital bleeding (anesthetized or unanesthetized)
  • Retro-orbital injections (performed on anesthetized mice only)
  • Decapitation on adult mice >14 days old (unanesthetized)
  • Tape skirt

Rats:

  • Cervical dislocation performed only on rats < 200 grams (anesthetized or unanesthetized)
  • Retro-orbital bleeding (performed on anesthetized rats only)
  • Decapitation on adult rats >14 days old (unanesthetized)

Fish:

  • Stunning followed by caudal tail vein severing
  • Stunning followed by gill severing
  • Stunning followed by decapitation
  • Carbon dioxide (CO2) euthanasia

Amphibians (for appropriate species only):

  • Pithing

Birds:

  • Decapitation (unanesthetized)
  • Cervical dislocation (unanesthetized)

Rabbits:

  • Decapitation (unanesthetized)
  • Penetrating captive bolt pistol
  • Cervical dislocation performed only on rabbits < 1 Kg (anesthetized or unanesthetized)

Guinea Pigs:

  • Decapitation (unanesthetized)

Hamsters:

  • Decapitation (unanesthetized)
  • Retro-orbital bleeding (anesthetized or unanesthetized)

Gerbils:

  • Decapitation (unanesthetized)
  • Retro-orbital bleeding (anesthetized or unanesthetized)
  • Retro-orbital injections (performed on anesthetized gerbils only)

USDA-Covered Species (mammals except for mice and rats):

All Species: