Molecular and Genetics Core of the
Diabetes and Endocrinology Research Center
(DERC)



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Quantitative PCR - The MGC provides quantitative, or real-time PCR using a Stratagene Mx4000 quantitative PCR instrument.  The MGC also designs probes - TaqMan and MGB (minor groove binding) and primers for your gene of interest, and provides at no charge several probes for normalizing genes.  Three genes in the same reaction can be measured. Click here for more information.

Genotyping - Single Nucleotide Polymorphism (SNP) typing and allele discrimination. Click here for more information.

Quantitation of DNA and RNA - sensitive quantitation by fluorescent dyes. Click here for more information.

RNA Isolation - of total or poly A+ RNA from tissue cultured cells, tissues, or blood.

DNA Purification - genomic DNA from rat, mouse, or human tissues, or plasmid DNA.

Cloning - Cloning genes from cDNA into plasmid vectors, site-directed mutagenesis.

Sequencing - carry out DNA sequence reactions and obtain sequences.

Northern Blotting - prepare blot, hybridize with probe, image the results, and quantitate.

Consulting - assistance is provided in project design and analysis of results. 


 

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