Co-adsorbed fibrinogen and von Willebrand factor augment platelet procoagulant activity and spreading

Citation

Grunkemeier, J. M.; Tsai, W. B.; & Horbett, T. A. (2001). Co-adsorbed fibrinogen and von Willebrand factor augment platelet procoagulant activity and spreading. J Biomater Sci Polym Ed, 12(1), 1-20.

Abstract

Previously we observed that platelets adherent to surfaces preadsorbed with blood plasma exhibited 1.3 to 2.4 times greater procoagulant activity than platelets on surfaces adsorbed with fibrinogen (Fg) only. These observations suggested that the adhesion proteins adsorbed from plasma may activate platelets in a cooperative, or synergistic manner. In the present study, polystyrene surfaces adsorbed with both Fg and vWF induced up to three times greater procoagulant activity than surfaces adsorbed with Fg or vWF only. The amounts of Fg and vWF adsorbed from binary mixtures that resulted in increased procoagulant activity were found to be similar to the amounts that adsorbed to PS from 100% plasma. The effect of adsorbed adhesion proteins on platelet spreading was also investigated. The proportion of fully spread platelets increased, depending on the adhesion protein preadsorbed to the surface, in the following order: vWF < Fg < Fn < (vWF + Fg) < Vn < plasma.

Keyword(s)

Blood Platelets
Cell Adhesion
Cell Movement
Coagulants
Dose-Response Relationship, Drug
Fibrinogen
Humans
L-Lactate Dehydrogenase
Microscopy, Electron, Scanning
Plasma
Platelet Aggregation
Polystyrenes
Thromboplastin
von Willebrand Factor

Reference Type

Journal Article

Secondary Title

J Biomater Sci Polym Ed

Author(s)

Grunkemeier, J. M.
Tsai, W. B.
Horbett, T. A.

Year Published

2001

Date Published

978307200

Volume Number

12

Issue Number

1

Pages

1-20