Citation
Wu, Peng; Castner, David G.; & Grainger, David W. (2008).
Diagnostic devices as biomaterials: a review of nucleic acid and protein microarray surface performance issues.
J Biomater Sci Polym Ed, 19(6), 725-753.
Abstract
This review of current DNA and protein microarray diagnostic and bio-analytical technologies focuses on the different surface chemistries used in these miniaturized surface-capture formats. Description of current strategies in bio-immobilization and coupling to create multiplexed affinity bioassays in micrometer-sized printed spots, problems with current formats and review of some detection methods are included. Recommendations for improving long-standing challenges in DNA- and protein-based arrays are forwarded. The biomaterials community can contribute relevant expertise to these formidable bio-interfacial problems that represent significant barriers to clinical implementation of microarray assays.
Keyword(s)
Molecular Diagnostic TechniquesOligonucleotide Array Sequence AnalysisProtein Array Analysis
Reference Type
Journal Article
Secondary Title
J Biomater Sci Polym Ed
Author(s)
Wu, PengCastner, David G.Grainger, David W.
Year Published
2008
Date Published
1199145600
Volume Number
19
Issue Number
6
Pages
725-753
DOI
Diagnostic devices as biomaterials10.1163/156856208784522092
