Citation
Barnes, C. A.; Brison, J.; Robinson, M.; Graham, D. J.; Castner, D. G.; & Ratner, B. D. (2012). Identifying Individual Cell Types in Heterogeneous Cultures Using Secondary Ion Mass Spectrometry Imaging with C-60 Etching and Multivariate Analysis. Anal Chem, 84(2), 893-900.Abstract
Tissue engineering approaches fabricate and subsequently, implant cell-seeded and unseeded scaffold biomaterials. Once in the body, these biomaterials are repopulated with somatic cells of various phenotypes whose identification upon explantation can be expensive and time-consuming. We show that imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) can be used to distinguish mammalian cell types in heterogeneous cultures. Primary rat esophageal epithelial cells (REEC) were cultured with NIH 3T3 mouse fibroblasts on tissue culture polystyrene and freeze-dried before TOF-SIMS imaging. Results show that a short etching sequence with C-60(+) can be used to clean the sample surface and improve the TOF-SIMS image quality. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) were used to identify peaks whose contributions to the total variance in the multivariate model Were due to either the two cell types or the substrate. Using PLS-DA, unknown regions of cellularity that were otherwise unidentifiable by SIMS could be classified. From the loadings in the PLS-DA model, peaks were selected that were indicative of the two cell types and TOF-SIMS images were created and overlaid that showed the ability of this method to distinguish features visually..Keyword(s)
adsorbed protein filmsbuckminsterfullereneclassificationmatrixmembranessamplessingle cellsspatial-resolutiontissuetof-simsNotes
876UYTimes Cited:8
Cited References Count:48