In vitro characterization of peptide-modified p(AAm-co-EG/AAc) IPN-coated titanium implants

Citation

Barber, Thomas A.; Gamble, Lara J.; Castner, David G.; & Healy, Kevin E. (2006). In vitro characterization of peptide-modified p(AAm-co-EG/AAc) IPN-coated titanium implants. Journal of orthopaedic research: official publication of the Orthopaedic Research Society, 24(7), 1366-1376.

Abstract

Interpenetrating polymer networks (IPNs) of poly(acrylamide-co-ethylene glycol/acrylic acid) [p(AAm-co-EG/AAc)] functionalized with an -Arg-Gly-Asp- containing peptide derived from rat bone sialoprotein [bsp-RGD(15)] were grafted to titanium implants in an effort to modulate osteoblast behavior in vitro. Surface characterization data were consistent with the presence of an IPN, and ligand density measurements established that the range of peptide density on the modified implants spanned three orders of magnitude (0.01-20 pmol/cm2). In vitro biological characterization of the modified implants employing the primary rat calvarial osteoblast (RCO) model resulted in the identification of a critical ligand density (0.01

Keyword(s)

Acrylic Resins
Animals
Cell Differentiation
Cells, Cultured
Coated Materials, Biocompatible
Implants, Experimental
Integrin-Binding Sialoprotein
Osteoblasts
Peptides
Polyethylene Glycols
Rats
Sialoglycoproteins
Spectroscopy, Electron Energy-Loss
Titanium

Reference Type

Journal Article

Secondary Title

Journal of orthopaedic research: official publication of the Orthopaedic Research Society

Author(s)

Barber, Thomas A.
Gamble, Lara J.
Castner, David G.
Healy, Kevin E.

Year Published

2006

Volume Number

Issue Number

Pages

1366-1376

DOI

10.1002/jor.20165