Citation
Michel, Roger; Pasche, Stephanie; Textor, Marcus; & Castner, David G. (2005).
Influence of PEG Architecture on Protein Adsorption and Conformation.
Langmuir, 21(26), 12327-12332.
Abstract
Poly(l-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) copolymers with various grafting ratios were adsorbed to niobium pentoxide-coated silicon wafers and characterized before and after protein adsorption using X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF?SIMS). Three proteins of different sizes, myoglobin (16 kD), albumin (67 kD), and fibrinogen (340 kD), were studied. XPS was used to quantify the amount of protein adsorbed to the bare and PEGylated surfaces. ToF?SIMS and principal component analysis (PCA) were used to study protein conformational changes on these surfaces. The smallest protein, myoglobin, generally adsorbed in higher numbers than the much larger fibrinogen. Protein adsorption was lowest on the surfaces with the highest PEG chain surface density and increased as the PEG layer density decreased. The highest adsorption was found on lysine-coated and bare niobium surfaces. ToF?SIMS and PCA data evaluation provided further information on the degree of protein denaturation, which, for a particular protein, were found to decrease with increasing PEG surface density and increase with decreasing protein size.
Reference Type
Journal Article
Secondary Title
Langmuir
Author(s)
Michel, RogerPasche, StephanieTextor, MarcusCastner, David G.
Year Published
2005
Date Published
1133395200
Volume Number
21
Issue Number
26
Pages
12327-12332
DOI
10.1021/la051726h
