Interkingdom Signaling: Integration, Conformation, and Orientation of N-Acyl-l-homoserine Lactones in Supported Lipid Bilayers

Citation

Barth, Christoph; Jakubczyk, Dorota; Kubas, Adam; Anastassacos, Frances; Brenner-Weiss, Gerald; Fink, Karin; Schepers, Ute; Bräse, Stefan; & Koelsch, Patrick (2012). Interkingdom Signaling: Integration, Conformation, and Orientation of N-Acyl-l-homoserine Lactones in Supported Lipid Bilayers. Langmuir, 28(22), 8456-8462.

Abstract

N-Acyl-l-homoserine lactones (AHLs) are small cell-to-cell signaling molecules involved in the regulation of population density and local gene expression in microbial communities. Recent evidence shows that contact of this signaling system, usually referred to as quorum sensing, to living eukaryotes results in interactions of AHL with host cells in a process termed ?interkingdom signaling?. So far details of this process and the binding site of the AHLs remain unknown; both an intracellular and a membrane-bound receptor seem possible, the first of which requires passage through the cell membrane. Here, we used sum-frequency-generation (SFG) spectroscopy to investigate the integration, conformation, orientation, and translocation of deuterated N-acyl-l-homoserine lactones (AHL-dn) with varying chain length (8, 12, and 14 C atoms) in lipid bilayers consisting of a 1:1 mixture of POPC:POPG supported on SiO2 substrates (prepared by vesicle fusion). We found that all AHL-dn derivatives are well-ordered within the supported lipid bilayer (SLB) in a preferentially all-trans conformation of the deuterated alkyl chain and integrated into the upper leaflet of the SLB with the methyl terminal groups pointing downward. For the bilayer system described above, no flip-flop of AHL-dn from the upper leaflet to the lower one could be observed. Spectral assignments and interpretations were further supported by Fourier transform infrared and Raman spectroscopy.

Reference Type

Journal Article

Secondary Title

Langmuir

Author(s)

Barth, Christoph
Jakubczyk, Dorota
Kubas, Adam
Anastassacos, Frances
Brenner-Weiss, Gerald
Fink, Karin
Schepers, Ute
Bräse, Stefan
Koelsch, Patrick

Year Published

2012

Date Published

1338854400

Volume Number

28

Issue Number

22

Pages

8456-8462

DOI

Interkingdom Signaling10.1021/la301241s