Micro-Scale Cell Patterning on Nonfouling Plasma Polymerized Tetraglyme Coatings by Protein Microcontact Printing

Citation

Pan, Y. Vickie; McDevitt, Todd C.; Kim, Tae Kyun; Leach-Scampavia, Deborah; Stayton, Patrick S.; Denton, Denice D.; & Ratner, Buddy D. (2002). Micro-Scale Cell Patterning on Nonfouling Plasma Polymerized Tetraglyme Coatings by Protein Microcontact Printing. Plasmas and Polymers, 7(2), 171-183.

Abstract

Nonfouling thin films were prepared by the plasma deposition of tetraethylene glycol dimethyl ether (pp4G) on fluorinated ethylene propylene polymer (FEP) and glass substrates. Ordered cell patterns were created on these surfaces by microcontact printing of proteins. Pp4G was found to be stable in aqueous environments and resistant to an ethanol sterilization procedure, as verified by surface analysis. Pp4G also reduced nonspecific protein adsorption by more than 65-fold before and after sterilization. Despite the low adsorption of proteins to pp4G in solution, protein microcontact printing was achieved and we were able to print laminin, an adhesive extracellular matrix protein, from an elastomeric stamp onto pp4G. The printed laminin supported the attachment and spreading of cardiomyocytes and the nonprinted pp4G regions remained cell repulsive in culture conditions. Microscale patterns of cardiomyocytes were maintained on printed pp4G for more than 7 days. This cell patterning process should be viable for other cell types. The potential applications include tissue engineering and microdevices for biosensor, diagnostic, and pharmacological applications.

Keyword(s)

cell patterning
Characterization and Evaluation Materials
Inorganic Chemistry
Mechanical Engineering
Mechanics
Nonfouling
Nuclear Physics, Heavy Ions, Hadrons
plasma polymerization
protein microcontact printing
surface analysis

Reference Type

Journal Article

Secondary Title

Plasmas and Polymers

Author(s)

Pan, Y. Vickie
McDevitt, Todd C.
Kim, Tae Kyun
Leach-Scampavia, Deborah
Stayton, Patrick S.
Denton, Denice D.
Ratner, Buddy D.

Year Published

2002

Date Published

1022889600

Volume Number

Issue Number

Pages

171-183

ISSN/ISBN

1084-0184, 1572-8978

DOI

10.1023/a:1016295419941