Citation
Wang, H.; Castner, D. G.; Ratner, B. D.; & Jiang, S. Y. (2004). Probing the orientation of surface-immobilized immunoglobulin G by time-of-flight secondary ion mass spectrometry. Langmuir, 20(5), 1877-1887.Abstract
Static time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a powerful surface analysis technique for the characterization of protein films because of its chemical selectivity and surface sensitivity. In this study, static ToF-SIMS and principal component analysis (PCA), a multivariate data analysis method, were combined to probe the orientation of surface-immobilized immunoglobulin G (IgG). IgG orientation can enhance its ability to detect its antigen in immunoassay techniques. The IgG used in this work is the mouse monoclonal anti-human chorionic gonadotropin (anti-hCG). Anti-hCG films on different well-defined substrates have been studied using its F(ab')(2) and Fc fragments as references. Atomic force microscopy was used to characterize these protein films before static ToF-SIMS analysis. The results from PCA of ToF-SIMS spectra were related to the antibody primary amino acid composition and its three-dimensional structure.Keyword(s)
adsorbed protein filmsAdsorptionelectrolyte-solutionsforce microscopefragmentsgold surfacesimmunosensororiented immobilizationprincipal component analysisself-assembled monolayersNotes
778KVTimes Cited:80
Cited References Count:36