Protein contact printing for a surface plasmon resonance biosensor with on-chip referencing

Citation

Lu, Hongbo B.; Homola, Jiřı́; Campbell, Charles T.; Nenninger, Garet G.; Yee, Sinclair S.; & Ratner, Buddy D. (2001). Protein contact printing for a surface plasmon resonance biosensor with on-chip referencing. Sensors and Actuators B: Chemical, 74(1–3), 91-99.

Abstract

Protein contact printing (pCP) has been applied to locally functionalize a novel wavelength-modulated single flow channel surface plasmon resonance (SPR) sensor with an on-chip reference. The SPR sensor has a high refractive index dielectric tantalum pentoxide (Ta2O5) overlayer covering part of the gold sensing surface to generate a SPR resonance (SPR-Ta) that is spectrally-separated from the resonance of the Ta2O5-free Au resonance (SPR-Au). Thus, an on-chip reference channel is provided [Electron. Lett. 35 (1999) 1105]. This communication demonstrates that pCP can be used for functionalizing such dual-channel SPR sensors. By using pCP, bovine serum albumin (BSA) was shown to passivate the surfaces well enough to prevent non-specific protein adsorption. In contrast, the printed 2,4-dinitrophenylated BSA (DNP-BSA) was recognized specifically by anti-dinitrophenyl antibodies (α-DNP) in solution. By printing DNP-BSA and BSA onto the Au and Ta2O5 surfaces, respectively, we demonstrate that the reversible bulk refractive index changes presented in both signals can be canceled out. Therefore, a more accurate binding curve for the α-DNP/DNP interaction can be obtained by subtracting the properly scaled SPR-Ta reference signal from the SPR-Au signal. We show that pCP is a simple, efficient, and versatile method for delivering multiple proteins with sufficient surface coverage and activity onto such sensor surfaces without cross-interference to adjacent areas.

Keyword(s)

Biosensors
Protein contact printing
Protein patterning
Surface plasmon resonance (SPR)

Reference Type

Journal Article

Secondary Title

Sensors and Actuators B: Chemical

Author(s)

Lu, Hongbo B.
Homola, Jiřı́
Campbell, Charles T.
Nenninger, Garet G.
Yee, Sinclair S.
Ratner, Buddy D.

Year Published

2001

Date Published

987292800

Volume Number

Issue Number

1–3

Pages

91-99

DOI

10.1016/s0925-4005(00)00716-4