Citation
Taylor, A. J.; Ratner, B. D.; Buttery, L. D. K.; & Alexander, M. R. (2015). Revealing cytokine-induced changes in the extracellular matrix with secondary ion mass spectrometry. Acta Biomaterialia, 14, 70-83.Abstract
Cell-secreted matrices (CSMs), where extracellular matrix (ECM) deposited by monolayer cell cultures is decellularized, have been increasingly used to produce surfaces that may be reseeded with cells. Such surfaces are useful to help us understand cell-ECM interactions in a microenvironment closer to the in vivo situation than synthetic substrates with adsorbed proteins. We describe the production of CSMs from mouse primary osteoblasts (mPObs) exposed to cytokine challenge during matrix secretion, mimicking in vivo inflammatory environments. Time-of-flight secondary ion mass spectrometry data revealed that CSMs with cytokine challenge at day 7 or 12 of culture can be chemically distinguished from one another and from untreated CSM using multivariate analysis. Comparison of the differences with reference spectra from adsorbed protein mixtures points towards cytokine challenge resulting in a decrease in collagen content. This is supported by immunocytochemical and histological staining, demonstrating a 44% loss of collagen mass and a 32% loss in collagen I coverage. CSM surfaces demonstrate greater cell adhesion than adsorbed ECM proteins. When mPObs were reseeded onto cytokine-challenged CSMs they exhibited reduced adhesion and elongated morphology compared to untreated CSMs. Such changes may direct subsequent cell fate and function, and provide insights into pathological responses at sites of inflammation. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.Keyword(s)
adsorbed protein filmscollagen-synthesisECM (extracellular matrix)Inflammationmesenchymal stem-cellsmultivariate-analysisnecrosis-factor-alphaosteoblastosteoblast differentiationosteogenic differentiationprincipal component analysisproteomic analysisSIMSsurface analysistof-sims analysisNotes
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