Secondary Ion Mass Spectrometry Imaging of Tissues, Cells, and Microbial Systems

Citation

Gamble, L. J. & Anderton, C. R. (2016). Secondary Ion Mass Spectrometry Imaging of Tissues, Cells, and Microbial Systems. Micros Today, 24(2), 24-31. PMCID: 5028133

Abstract

Secondary ion mass spectrometry (SIMS) is a technique capable of imaging tissues, single cells, and microbes revealing chemical species with sub-micrometer spatial resolution. The recently developed Fourier transform ion cyclotron resonance (FTICR) SIMS instrument provides high mass resolving power and mass accuracy, ToF-SIMS can generate chemical maps with an order of magnitude better lateral resolution than the FTICR-SIMS, and the NanoSIMS instrument offers sub-100 nm spatial resolution in chemical imaging. Many commercial ToF-SIMS instruments are also capable of depth profiling that allows three-dimensional reconstructions of cell and tissue structure.

Notes

Gamble, Lara J
Anderton, Christopher R
P41 EB002027/EB/NIBIB NIH HHS/
Micros Today. 2016 Mar;24(2):24-31. Epub 2016 Mar 18.

Reference Type

Journal Article

Secondary Title

Micros Today

Author(s)

Gamble, L. J.
Anderton, C. R.

Year Published

2016

Date Published

1456790400

Volume Number

24

Issue Number

2

Pages

24-31

ISSN/ISBN

1551-9295 (Print)
1551-9295 (Linking)

DOI

10.1017/S1551929516000018

PMCID

5028133