Citation
Tegoulia, Vassiliki A. & Cooper, Stuart L. (2002).
Staphylococcus aureus adhesion to self-assembled monolayers: effect of surface chemistry and fibrinogen presence.
Colloids and Surfaces B: Biointerfaces, 24(3–4), 217-228.
Abstract
Staphylococcus aureus adhesion on self-assembled monolayers (SAMs) formed by the adsorption of alkanethiols on transparent gold films has been studied in real time under well-defined flow conditions using a radial flow chamber and an automated videomicroscopy system. SAMs terminated with methyl, hydroxyl, carboxylic acid and tri(ethylene oxide) groups were investigated. SAMs were characterized using contact angle measurements, ellipsometry and X-ray photoelectron spectroscopy. Adhesion experiments using the Newman strain of S. aureus were performed on bare monolayers and monolayers pre-incubated with fibrinogen. Adhesion was found to be lowest on the ethylene oxide-bearing surfaces, followed by the hydroxyl surfaces. Adhesion on the carboxylic- and methyl-terminated SAMs was much higher. Bacterial adhesion was higher on the hydrophobic surfaces. Pre-incubation of surfaces with fibrinogen minimized the effect of the surface properties of the substrate. Adhesion was increased on all surfaces when fibrinogen was present and no significant differences were observed between adhesion to the different SAMs. This study showed that surfaces rich in ethylene oxide groups can be effectively used to prevent bacterial adhesion. However, under physiological conditions, most of the substrate properties are masked by the presence of the adsorbed protein layer and the effect of substrate properties on bacteria adhesion under flow is minimal.
Keyword(s)
Automated videomicroscopyBacterial adhesionself-assembled monolayersStaphylococcus aureusWell-defined flow field
Reference Type
Journal Article
Secondary Title
Colloids and Surfaces B: Biointerfaces
Author(s)
Tegoulia, Vassiliki A.Cooper, Stuart L.
Year Published
2002
Volume Number
24
Issue Number
3–4
Pages
217-228
DOI
Staphylococcus aureus adhesion to self-assembled monolayers10.1016/s0927-7765(01)00240-5
