Surface characterization of extracellular matrix scaffolds

Citation

Brown, B. N.; Barnes, C. A.; Kasick, R. T.; Michel, R.; Gilbert, T. W.; Beer-Stolz, D.; Castner, D. G.; Ratner, B. D.; & Badylak, S. F. (2010). Surface characterization of extracellular matrix scaffolds. Biomaterials, 31(3), 428-437. PMCID: 2783670

Abstract

Extracellular matrix (ECM) scaffolds prepared from different tissue sources or using different methods have been demonstrated to have distinctive effects upon cell adhesion patterns and the ability to support and maintain differentiated phenotypes. It is unknown whether the molecular composition or the ultrastructure of the ECM plays a greater role in determining the phenotype of the cells with which it comes into contact. However, when implanted, the topology and ligand landscape of the material will determine the host molecules that bind and the type and behavior of cells that mediate the host response. Therefore, a comprehensive understanding of surface characteristics is essential in the design of scaffolds for specific clinical applications. The surface characteristics of ECM scaffolds derived from porcine urinary bladder, small intestine, and liver as well as the effects of two commonly used methods of chemical cross-linking upon UBM were investigated. Electron microscopy and time of flight secondary ion mass spectroscopy were used to examine the surface characteristics of the scaffolds. The results show that ECM scaffolds have unique morphologic and structural properties which are dependant on the organ or tissue from which the scaffold is harvested. Furthermore, the results show that the surface characteristics of an ECM scaffold are changed through chemical cross-linking.

Keyword(s)

*Extracellular Matrix/chemistry/metabolism
Animals
Biocompatible Materials/chemistry/metabolism
Cells, Cultured
Cross-Linking Reagents/chemistry
Intestinal Mucosa/cytology
Intestine, Small/cytology
Liver/cytology
Mass Spectrometry/methods
Materials Testing
Phenotype
principal component analysis
Surface Properties
Swine
Tissue Engineering/*instrumentation/methods
Tissue Scaffolds/*chemistry
Urinary Bladder/cytology

Notes

Brown, Bryan N
Barnes, Christopher A
Kasick, Rena T
Michel, Roger
Gilbert, Thomas W
Beer-Stolz, Donna
Castner, David G
Ratner, Buddy D
Badylak, Stephen F
EB-002027/EB/NIBIB NIH HHS/
F31 EB007914-01A2/EB/NIBIB NIH HHS/
P41 EB002027/EB/NIBIB NIH HHS/
P41 EB002027-19/EB/NIBIB NIH HHS/
P41 EB002027-20/EB/NIBIB NIH HHS/
P41 EB002027-21/EB/NIBIB NIH HHS/
P41 EB002027-22/EB/NIBIB NIH HHS/
P41 EB002027-23/EB/NIBIB NIH HHS/
P41 EB002027-24/EB/NIBIB NIH HHS/
R01 AR054940-01/AR/NIAMS NIH HHS/
R01 AR054940-02/AR/NIAMS NIH HHS/
R01 AR054940-03/AR/NIAMS NIH HHS/
England
Biomaterials. 2010 Jan;31(3):428-37. doi: 10.1016/j.biomaterials.2009.09.061. Epub 2009 Oct 13.

Reference Type

Journal Article

Secondary Title

Biomaterials

Author(s)

Brown, B. N.
Barnes, C. A.
Kasick, R. T.
Michel, R.
Gilbert, T. W.
Beer-Stolz, D.
Castner, D. G.
Ratner, B. D.
Badylak, S. F.

Year Published

2010

Date Published

1737849600

Volume Number

31

Issue Number

3

Pages

428-437

ISSN/ISBN

1878-5905 (Electronic)
0142-9612 (Linking)

DOI

10.1016/j.biomaterials.2009.09.061

PMCID

2783670