Time of flight secondary ion mass spectrometry-A method to evaluate plasma-modified three-dimensional scaffold chemistry

Citation

Taylor, M. J.; Aitchison, H.; Hawker, M. J.; Mann, M. N.; Fisher, E. R.; Graham, D. J.; & Gamble, L. J. (2018). Time of flight secondary ion mass spectrometry-A method to evaluate plasma-modified three-dimensional scaffold chemistry. Biointerphases, 13(3), 03B415. PMCID: 5878104

Abstract

Biopolymers are used extensively in the manufacture of porous scaffolds for a variety of biological applications. The surfaces of these scaffolds are often modified to encourage specific interactions such as surface modification of scaffolds to prevent fouling or to promote a cell supportive environment for tissue engineering implants. However, few techniques can effectively characterize the uniformity of surface modifications in a porous scaffold. By filling the scaffold pores through polymer embedding, followed by analysis with imaging time-of-flight secondary ion mass spectrometry (ToF-SIMS), the distribution and composition of surface chemical species though complex porous scaffolds can be characterized. This method is demonstrated on poly(caprolactone) scaffolds modified with a low-fouling plasma-deposited coating from octafluoropropane via plasma enhanced chemical vapor deposition. A gradient distribution of CF(+)/CF3(+) is observed for scaffolds plasma treated for 5 min, whereas a 20 min treatment results in more uniform distribution of the surface modification throughout the entire scaffold. The authors expect this approach to be widely applicable for ToF-SIMS analysis of scaffolds modified by multiple plasma processing techniques as well as alternative surface modification approaches.

Keyword(s)

*Plasma Gases
*Surface Properties
Biopolymers/*analysis
Fluorocarbons/*analysis
Polyesters/*analysis
Spectrometry, Mass, Secondary Ion/*methods
Tissue Scaffolds/*chemistry

Notes

Taylor, Michael J
Aitchison, Hannah
Hawker, Morgan J
Mann, Michelle N
Fisher, Ellen R
Graham, Daniel J
Gamble, Lara J
P41 EB002027/EB/NIBIB NIH HHS/
Biointerphases. 2018 Mar 30;13(3):03B415. doi: 10.1116/1.5023005.

Reference Type

Journal Article

Secondary Title

Biointerphases

Author(s)

Taylor, M. J.
Aitchison, H.
Hawker, M. J.
Mann, M. N.
Fisher, E. R.
Graham, D. J.
Gamble, L. J.

Year Published

2018

Date Published

1743292800

Volume Number

Issue Number

ISSN/ISBN

1559-4106 (Electronic)
1559-4106 (Linking)

DOI

10.1116/1.5023005

PMCID

5878104