Alliance for Pandemic Preparedness

April 10, 2020

Optimization of SARS-CoV-2 detection by RT-QPCR without RNA extraction

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  • The authors evaluated a direct RT-QPCR method without RNA extraction and the influence of swab storage media on detection.  Their SeeGene’s assay provided similar efficiency to RealStar®.
  • The authors conclude that RNA extraction may not be necessary if samples are stored in UTM or molecular water; and also recommend that samples be stored in saline solution or Hanks medium.

Merindol et al. (Apr 10, 2020). Optimization of SARS-CoV-2 detection by RT-QPCR without RNA extraction. Pre-print downloaded Apr 10 from https://doi.org/10.1101/2020.04.06.028902