Alliance for Pandemic Preparedness
April 10, 2020
Optimization of SARS-CoV-2 detection by RT-QPCR without RNA extraction
Category: Article Summary
Topic: Testing and Treatment
- The authors evaluated a direct RT-QPCR method without RNA extraction and the influence of swab storage media on detection. Their SeeGene’s assay provided similar efficiency to RealStar®.
- The authors conclude that RNA extraction may not be necessary if samples are stored in UTM or molecular water; and also recommend that samples be stored in saline solution or Hanks medium.
Merindol et al. (Apr 10, 2020). Optimization of SARS-CoV-2 detection by RT-QPCR without RNA extraction. Pre-print downloaded Apr 10 from https://doi.org/10.1101/2020.04.06.028902