Alliance for Pandemic Preparedness

June 8, 2020

Identification of Nsp1 Gene as the Target of SARS-CoV-2 Real-Time RT-PCR Using Nanopore Whole Genome Sequencing

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  • Chan et al. found that SARS-CoV-2 mutations in current binding sites for PCR tests may compromise their sensitivity and identified the nsp1 gene as a novel target. An RT-PCR assay developed using this target had a sensitivity of 93.1% and a specificity of 100%.   

Chan et al. (June 2020). Identification of Nsp1 Gene as the Target of SARS-CoV-2 Real-Time RT-PCR Using Nanopore Whole Genome Sequencing. Journal of Medical Virology. https://doi.org/10.1002/jmv.26140