Alliance for Pandemic Preparedness

June 18, 2020

Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of SARS-CoV-2

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  • Lau et al. developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of SARS-CoV-2. In comparison to the RT-qPCR assay, the investigators reported that the RT-LAMP assay was 2.5 times faster and had a clinical sensitivity and specificity of 100%. However, the RT-LAMP assay had a 5-fold higher limit of detection,  

Lau et al. (June 3, 2020). Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification for Rapid Detection of SARS-CoV-2. PeerJ. https://doi.org/10.7717/peerj.9278